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Research Article
Structure of an intermediate in the unfolding of creatine kinase
Article first published online: 7 DEC 2000
DOI: 10.1002/1097-0134(20010201)42:2<269::AID-PROT140>3.0.CO;2-Y
Copyright © 2000 Wiley-Liss, Inc.
Issue
1097-0134/asset/cover.gif?v=1&s=d817e79b67ba6cacf8bdcce1a819c04de300a7e3 "Proteins: Structure, Function, and Bioinformatics")
Proteins: Structure, Function, and Bioinformatics
Volume 42, Issue 2, pages 269–278, 1 February 2001
Additional Information
How to Cite
Webb, T. I. and Morris, G. E. (2001), Structure of an intermediate in the unfolding of creatine kinase. Proteins, 42: 269–278. doi: 10.1002/1097-0134(20010201)42:2<269::AID-PROT140>3.0.CO;2-Y
Publication History
- Issue published online: 7 DEC 2000
- Article first published online: 7 DEC 2000
- Manuscript Accepted: 28 SEP 2000
- Manuscript Received: 1 JUN 2000
- Abstract
- Article
- References
- Cited By
Keywords:
- protein folding;
- domain structure;
- antibody;
- protease;
- epitope;
- guanidinium;
- molten globule;
- dimer
Abstract
The homodimeric muscle isoform of creatine kinase (MM-CK) unfolds on exposure to low levels of guanidinium chloride (GdmCl) to yield a partly folded monomeric intermediate. Those regions of MM-CK that experience local unfolding were previously identified through an extensive study of antibody accessibility and protease sensitivity. Since these studies were completed, the coordinates of the rabbit isoform (MM-CK) were released. In light of this, we have determined the minimum changes to this structure required to explain our data on protease and epitope accessibility in the intermediate. We propose that the observed changes occur through (a) disruption of the monomer-monomer interface during dissociation, (b) separation and/ or unfolding of domains or subdomains, and (c) the partial unfolding of solvent-exposed helices. The proposed structure for the intermediate is consistent both with current models of unfolding intermediates and the results of independent studies pertaining to the unfolding of creatine kinase. Proteins 2001;42:269–278. © 2000 Wiley-Liss, Inc.