Characterization of a CD34+ cell line established from a child with large cell cutaneous lymphoma

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Abstract

Background. Lymphoma presenting with skin involvement has heterogeneous morphology and rarely is seen in children. To study the pathogenesis of this disease, lymphoma cells from a child with B-cell large cell lymphoma of the skin were cultured in vitro.

Methods. Lymphoma cells cultured on a feeder layer under hypoxic conditions grew in vitro after a latency period of 2 weeks. Since interleukin-6 (IL-6) induces final differentiation of activated B-lymphocytes, the cell line was evaluated for the presence of IL-6 receptors and biologic response to IL-6.

Results. An Epstein–Barr virus (EBV)-negative cell line (UoC-B2) was established which expressed CD34, CD45, HLA-DR, CD19, CD20, sIgM, sIgD, and lambda light chain. Good general concordance was observed between the patient's lymphoma and the cell line by comparing the immunophenotype, genotype, and karyotype. The UoC-B2 cells expressed surface IgM but did not secrete IgM into the culture media even in the presence of supplemental IL-6.

Conclusions. A B-lymphoid cell line (UoC-B2) was established from a child with primary cutaneous lymphoma. The cells expressed cell surface IgM and receptors for IL-6 but supplemental IL-6 had no effect on IgM production or cell proliferation.

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