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Original Article
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Preoperative diagnosis of intraductal papillary-mucinous tumors of the pancreas with attention to telomerase activity
Article first published online: 4 JAN 2001
DOI: 10.1002/1097-0142(20010101)91:1<35::AID-CNCR5>3.0.CO;2-A
Copyright © 2001 American Cancer Society
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How to Cite
Inoue, H., Tsuchida, A., Kawasaki, Y., Fujimoto, Y., Yamasaki, S. and Kajiyama, G. (2001), Preoperative diagnosis of intraductal papillary-mucinous tumors of the pancreas with attention to telomerase activity. Cancer, 91: 35–41. doi: 10.1002/1097-0142(20010101)91:1<35::AID-CNCR5>3.0.CO;2-A
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Publication History
- Issue published online: 4 JAN 2001
- Article first published online: 4 JAN 2001
- Manuscript Accepted: 28 AUG 2000
- Manuscript Revised: 31 JUL 2000
- Manuscript Received: 4 APR 2000
- Abstract
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Keywords:
- intraductal papillary-mucinous tumor of the pancreas;
- telomerase activity;
- endoscopic retrograde pancreatic juice aspiration;
- malignant
Abstract
BACKGROUND
It has been reported that, in patients with intraductal papillary-mucinous tumor (IPMT) of the pancreas, it is difficult to distinguish adenoma from carcinoma preoperatively. Recently, it has also been reported that telomerase activity was detected in many patients with carcinoma. In this report, the authors used the method of telomerase repeat amplification protocol (TRAP) assay on pancreatic juice retrieved by endoscopic retrograde pancreatic juice aspiration (ERP aspiration).
METHODS
Pancreatic juice was collected from 28 patients (13 with intraductal carcinoma and 15 with adenoma) using ERP aspiration at either Hiroshima University Hospital or its affiliated hospitals. Two samples of pancreatic juice were collected from each patient. Each sample was examined by cytology for Papanicolaou staining and TRAP assay.
RESULTS
Four of 13 IPMT patients (31%) with intraductal carcinoma were diagnosed accurately by cytology. Seven of nine patients who were classified with benign tumors by cytologic assessment had tumors that expressed telomerase activity. Overall, 11 of 13 IPMT patients (85%) with intraductal carcinoma were diagnosed correctly by cytology associated with telomerase activity. All of the IPMT patients with adenoma were classified with benign tumors by cytologic assessment, and telomerase activity was not expressed.
CONCLUSIONS
In this study, the authors found that telomerase activity was expressed with a comparatively high probability in intraductal carcinoma. These results suggest that telomerase activity in pancreatic juices may be used as an adjunct to cytologic diagnosis and may aid further in distinguishing between benign IPMT and malignant IPMT of the pancreas preoperatively. Cancer 2001;91:35–41. © 2001 American Cancer Society.