Low-cost microbioreactor for high-throughput bioprocessing
Article first published online: 19 DEC 2000
Copyright © 2001 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 72, Issue 3, pages 346–352, 5 February 2001
How to Cite
Kostov, Y., Harms, P., Randers-Eichhorn, L. and Rao, G. (2001), Low-cost microbioreactor for high-throughput bioprocessing. Biotechnol. Bioeng., 72: 346–352. doi: 10.1002/1097-0290(20010205)72:3<346::AID-BIT12>3.0.CO;2-X
- Issue published online: 19 DEC 2000
- Article first published online: 19 DEC 2000
- Manuscript Accepted: 17 OCT 2000
- Manuscript Received: 1 JUL 2000
- Genetech, Merck, and Pfizer
- optical sensing;
The design of a microbioreactor is described. An optical sensing system was used for continuous measurements of pH, dissolved oxygen, and optical density in a 2 mL working volume. The KLa of the microbioreactor was evaluated under different conditions. An Escherichia coli fermentation in both the microbioreactor and a standard 1 L bioreactor showed similar pH, dissolved oxygen, and optical density profiles.%The low cost of the microbioreactor, detection system, and the small volume of the fermentation broth provide a basis for development of a multiple-bioreactor system for high-throughput bioprocess optimization. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 346–352, 2001.