Adult rat and human bone marrow stromal cells differentiate into neurons
Article first published online: 31 JUL 2000
Copyright © 2000 Wiley-Liss, Inc.
Journal of Neuroscience Research
Volume 61, Issue 4, pages 364–370, 15 August 2000
How to Cite
Woodbury, D., Schwarz, E. J., Prockop, D. J. and Black, I. B. (2000), Adult rat and human bone marrow stromal cells differentiate into neurons. J. Neurosci. Res., 61: 364–370. doi: 10.1002/1097-4547(20000815)61:4<364::AID-JNR2>3.0.CO;2-C
- Issue published online: 31 JUL 2000
- Article first published online: 31 JUL 2000
- Manuscript Accepted: 30 MAY 2000
- Manuscript Revised: 23 MAY 2000
- Manuscript Received: 31 MAR 2000
- mesenchymal stem cells;
- neuronal differentiation;
- neurological diseases
Bone marrow stromal cells exhibit multiple traits of a stem cell population. They can be greatly expanded in vitro and induced to differentiate into multiple mesenchymal cell types. However, differentiation to non-mesenchymal fates has not been demonstrated. Here, adult rat stromal cells were expanded as undifferentiated cells in culture for more than 20 passages, indicating their proliferative capacity. A simple treatment protocol induced the stromal cells to exhibit a neuronal phenotype, expressing neuron-specific enolase, NeuN, neurofilament-M, and tau. With an optimal differentiation protocol, almost 80% of the cells expressed NSE and NF-M. The refractile cell bodies extended long processes terminating in typical growth cones and filopodia. The differentiating cells expressed nestin, characteristic of neuronal precursor stem cells, at 5 hr, but the trait was undetectable at 6 days. In contrast, expression of trkA, the nerve growth factor receptor, persisted from 5 hr through 6 days. Clonal cell lines, established from single cells, proliferated, yielding both undifferentiated and neuronal cells. Human marrow stromal cells subjected to this protocol also differentiated into neurons. Consequently, adult marrow stromal cells can be induced to overcome their mesenchymal commitment and may constitute an abundant and accessible cellular reservoir for the treatment of a variety of neurologic diseases. J. Neurosci. Res. 61:364–370, 2000. © 2000 Wiley-Liss, Inc.