Research Article

Human acid ceramidase is overexpressed but not mutated in prostate cancer

  1. Ratnam S. Seelan1,
  2. Chiping Qian1,
  3. Akira Yokomizo2,
  4. David G. Bostwick1,
  5. David I. Smith1,
  6. Wanguo Liu1,*

Article first published online: 12 JUL 2000

DOI: 10.1002/1098-2264(2000)9999:9999<::AID-GCC1018>3.0.CO;2-E

Issue

Genes, Chromosomes and Cancer

Genes, Chromosomes and Cancer

Volume 29, Issue 2, pages 137–146, October 2000

Additional Information

How to Cite

Seelan, R. S., Qian, C., Yokomizo, A., Bostwick, D. G., Smith, D. I. and Liu, W. (2000), Human acid ceramidase is overexpressed but not mutated in prostate cancer. Genes, Chromosomes and Cancer, 29: 137–146. doi: 10.1002/1098-2264(2000)9999:9999<::AID-GCC1018>3.0.CO;2-E

Author Information

  1. 1

    Division of Experimental Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota

  2. 2

    Department of Urology Research, Mayo Clinic, Rochester, Minnesota

*Division of Experimental Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First St., SW, Rochester, MN 55905

Publication History

  1. Issue published online: 10 AUG 2000
  2. Article first published online: 12 JUL 2000
  3. Manuscript Accepted: 31 MAR 2000
  4. Manuscript Received: 7 MAR 2000

Funded by

  • NIH. Grant Number: CA4803
  • Department of Defense. Grant Number: DAMD-17-98-1-8522
  • Public Health Service. Grant Number: CA-09441-16

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Abstract

The human acid ceramidase gene, that causes Farber disease, is located in 8p22, a region frequently altered in several cancers, including prostate cancer. Acid ceramidase catalyzes the hydrolysis of ceramide, a potent lipid second messenger molecule that promotes apoptosis and inhibits cellular proliferation. It is not known whether this gene, or its expression, is altered in prostate cancer. Here, we report the structural organization of the human gene, its expression in human tissues, and the identification of several single nucleotide polymorphisms. No cancer-related mutations were found in the gene in a panel of prostate tumor DNAs analyzed, but increased expression was observed in prostate tumor tissues when compared with matched normals. This increase was observed in all three prostate tumor cell lines tested (DU145, LnCAP, and PC3) when compared to a BPH (benign prostatic hyperplasia) cell line and 15/36 prostate tumors. These results suggest that acid ceramidase may play an important role in prostate carcinogenesis. © 2000 Wiley-Liss, Inc.

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