Localization of a novel tumor metastasis suppressor region on the short arm of human chromosome 2
Article first published online: 1 JUN 2000
Copyright © 2000 Wiley-Liss, Inc.
Genes, Chromosomes and Cancer
Volume 28, Issue 3, pages 285–293, July 2000
How to Cite
Mashimo, T., Goodarzi, G., Watabe, M., Cuthbert, A. P., Newbold, R. F., Pai, S. K., Hirota, S., Hosobe, S., Miura, K., Bandyopadhyay, S., Gross, S. C. and Watabe, K. (2000), Localization of a novel tumor metastasis suppressor region on the short arm of human chromosome 2. Genes Chromosom. Cancer, 28: 285–293. doi: 10.1002/1098-2264(200007)28:3<285::AID-GCC6>3.0.CO;2-O
- Issue published online: 1 JUN 2000
- Article first published online: 1 JUN 2000
- Manuscript Accepted: 14 DEC 1999
- Manuscript Received:
- National Institute of Health. Grant Number: R15 CA67290 01
- American Lung Association of Illinois
- William E. McElory Foundation
- Illinois Department of Public Health
Much of the lethality of malignant neoplasms is attributable directly to their ability to develop secondary growths in organs at a distance from the primary tumor mass, whereas few patients die from their primary neoplasm. Little is known about the molecular mechanism of tumor metastasis, however, which is controlled by a variety of positive and negative factors. In the search for metastasis suppressor genes, we have used the microcell-mediated chromosome transfer method and a rat prostate tumor model in SCID mice. When human chromosome 2 was introduced into the highly metastatic rat prostatic tumor cell, AT6.1, the metastatic ability of this cell was significantly (>99%) decreased in animals. An STS-based PCR analysis for 8 hybrid clones indicates that the suppressor activity is located in the p25–22 region of the chromosome. Furthermore, the AT6.1 cell with human chromosome 2 showed a reduced ability to invade Matrigel, suggesting that the suppressor activity is involved in the step of tumor invasion during the progression of prostate cancer. We have also examined the status of the suppressor region on chromosome 2 in human prostate cancer specimens and found that this region was often lost in high-grade tumors. These results suggest that the putative suppressor gene on chromosome 2 is functionally involved in the progression of human prostate cancer. Genes Chromosomes Cancer 28:285–293, 2000. © 2000 Wiley-Liss, Inc.