Turbidimetric determination of orthophosphate in digested plant material by flow-injection analysis
Article first published online: 18 DEC 2000
DOI: 10.1002/1098-2728(2000)12:5<236::AID-LRA3>3.0.CO;2-L
Copyright © 2000 John Wiley & Sons, Inc.
Additional Information
How to Cite
Diniz, M.-C. T., Oliveira, A. F. and Fatibello-Filho, O. (2000), Turbidimetric determination of orthophosphate in digested plant material by flow-injection analysis. Lab. Robotics Autom., 12: 236–240. doi: 10.1002/1098-2728(2000)12:5<236::AID-LRA3>3.0.CO;2-L
Publication History
- Issue published online: 18 DEC 2000
- Article first published online: 18 DEC 2000
- Manuscript Received: 5 DEC 1999
Funded by
- PADCT/CNPq
- CNPq
- FAPESP
- CAPES
- Abstract
- References
- Cited By
Abstract
A simple, fast, and low-cost flow-injection system is proposed for the determination of orthophosphate in digested plant material. The determination is based on the precipitation reaction of orthophosphate with 0.05 mol L−1 zinc solution in 0.05 mol L−1 triethanolamine buffer (TEA/TEAH+, pH 6.0) solution containing 0.025% (w/v) poly-(vinylalcohol). The zinc orthophosphate suspension produced was directly measured at 410 nm. Under the best experimental conditions, orthophosphate was determinated in the concentration range from 5 to 60 mg P L−1, and the relative standard deviations were less than 1.6% for 10 and 20 mg P L−1 solutions (n = 9). The analytical frequency was 180 h−1. The results obtained using the proposed FIA procedure and those obtained using a standard spectrophotometric procedure agree at the 95% confidence level. © 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:236–240, 2000