Application of partial least squares calibration for multicomponents determination by flow injection–solid phase spectrophotometry

Authors

  • Fábio S. Oliveira,

    1. Instituto de Química, Universidade Federal da Bahia
    Search for more papers by this author
  • Leonardo S. G. Teixeira,

    1. Instituto de Química, Universidade Federal da Bahia
    Search for more papers by this author
  • Mauro Korn

    Corresponding author
    1. Instituto de Química, Universidade Federal da Bahia; and Departamento de Ciências Exatas e da Terra, Universidade do Estado da Bahia Estrada das Barreiras, sn, Narandiba, Cabula, CEP 41195-001, Salvador-BA, Brazil
    • Departamento de Ciências Exatas e da Terra, Universidade do Estado da Bahia Estrada das Barreiras, sn, Narandiba, Cabula, CEP 41195-001, Salvador-BA, Brazil
    Search for more papers by this author

Abstract

This article reports the use of solid phase spectrophotometry associated with flow injection technique for simultaneous determination of zinc and nickel with 1-(2-thiazolylazo)-2-naphthol (TAN) immobilized on a C18 bonded silica support in the concentration range from 0.30 to 1.0 μg mL −1 of both ions. The multicomponent analysis was implemented using the absorbance signals in different intervals of time in a flow injection analysis peak for each sample solution inserted into the analytical path. A partial least squares (PLS) algorithm for model construction was developed in QuickBasic 4.5 as a subrotine in order to assemble it in a data acquisition program, permitting the concentration estimation in real time. The proposed method was successfully applied to nickel and zinc determination in synthetic mixtures with analytical throughput of 43 samples per hour. The results obtained with the proposed method were compared with Inductively Coupled Plasma Atomic E Mission Spectrometry (ICP-AES) data, and the results agreed with 95% confidence level. © 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:305–311, 2000

Ancillary