Research Article

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Interactive 3-D image analysis and visualization techniques for FISH-labelled chromosomes in interphase nuclei

  1. Ike A Ikizyan,
  2. Stefan Burde,
  3. James F Leary

Article first published online: 19 JUN 2001

DOI: 10.1002/1361-6374(199403)2:1<41::AID-BIO4>3.0.CO;2-Z

Issue

Bioimaging

Bioimaging

Volume 2, Issue 1, pages 41–56, March 1994

Additional Information

How to Cite

Ikizyan, I. A., Burde, S. and Leary, J. F. (1994), Interactive 3-D image analysis and visualization techniques for FISH-labelled chromosomes in interphase nuclei. Bioimaging, 2: 41–56. doi: 10.1002/1361-6374(199403)2:1<41::AID-BIO4>3.0.CO;2-Z

Author Information

  1. Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY, USA

Publication History

  1. Issue published online: 19 JUN 2001
  2. Article first published online: 19 JUN 2001
  3. Manuscript Accepted: 17 FEB 1994
  4. Manuscript Received: 1 JUN 1993

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Keywords:

  • 3-D imaging;
  • image analysis;
  • confocal microscopy;
  • 3-D displays;
  • 3-D image processing;
  • fluorescence in situ hybridization;
  • chromosome analysis

Abstract

New simple and fast methods for 3-D depth perception and interaction in volume visualization and quantitative analysis of dual-fluorescence confocal microscopy images are presented. By integrating a 3-D display and an associated 3-D input device, a system has been developed which creates an autostereoscopic confocal microscopy workstation environment on an IBM-compatible personal computer. The system incorporates appropriate 3-D image processing algorithms and enables the user to interactively address subregions in the data volume to identify domains of interest for subsequent volume and distance measurements. In the analysis of chromosomes in interphase nuclei these capabilities are shown to be useful in finding distances between chromosome domains and their respective volumes.