| 1. | Enzymatic Analysis Methods |
| 1.1. | Introduction |
| 1.2. | Enzymes: Basic Kinetics |
| 1.3. | Enzyme Assays: Practical Aspects |
| 1.3.1. | General Considerations |
| 1.3.2. | Spectrometric Methods |
| 1.3.3. | Other Methods |
| 1.3.4. | Effects of pH, Buffer, Composition, and Temperature |
| 1.3.5. | Sources and Activity of Enzymes |
| 1.4. | Enzyme Assays: Determination of Substrates |
| 1.4.1. | General Considerations |
| 1.4.2. | Determination of Glucose |
| 1.4.3. | Determination of Ethanol |
| 1.5. | Enzyme Assays: Determination of Inhibitors |
| 1.5.1. | Inhibitors of Cholinesterase Enzymes |
| 1.5.2. | Other Inhibition Methods |
| 1.6. | Enzyme Assays: Determination of Activators |
| 1.6.1. | Determination of Metal Ions by Metalloenzymes |
| 1.6.2. | Other Activator Analyses |
| 1.7. | Immobilized Enzymes |
| 1.7.1. | Introduction |
| 1.7.2. | Properties of Immobilized Enzymes |
| 1.7.3. | Application of Immobilized Enzymes |
| 2. | Immunoassays in Analytical Chemistry |
| 2.1. | Introduction |
| 2.2. | Polyclonal, Monoclonal, and Recombinant Antibodies |
| 2.3. | Sample Conditioning |
| 2.4. | Immunoassays |
| 2.4.1. | Radioimmunoassay |
| 2.4.1.1. | Isotopic Dilution Radioimmunoassay |
| 2.4.1.2. | Immunoradiometric Assay (IRMA) |
| 2.4.2. | Nonisotopic Homogeneous Immunoassay |
| 2.4.2.1. | Latex Particle Agglutination Immunoassay |
| 2.4.2.2. | Enzyme-Multiplied Immunoassay Technique (EMIT) |
| 2.4.2.3. | Apoenzyme Reconstitution Immunoassay System (ARIS) |
| 2.4.2.4. | Fluorophore-Labeled Homogeneous Immunoassay |
| 2.4.2.5. | Homogeneous Fluorescence Polarization Immunoassay (FPIA) |
| 2.4.2.6. | Homogeneous Liposome Immunoassay |
| 2.4.3. | Nonisotopic Heterogeneous Immunoassay |
| 2.4.3.1. | Enzyme-Linked Immunosorbent Assay (ELISA) |
| 2.4.3.2. | Sandwich Immunoassay |
| 2.4.3.3. | Immunoassays using Immobilized Affinity Ligands |
| 2.4.3.4. | Immunoassays with Biotin and Avidin |
| 2.4.3.5. | Magnetic Particle Immunoassay |
| 2.5. | Immunoaffinity Techniques |
| 2.6. | Immunoassays on Test Strips and other Planar Structures |
| 2.7. | Characterization and Interference |
| 2.8. | Future Immunological Applications and Techniques |
