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Enzyme and Immunoassays

  1. James N. Miller1,
  2. Reinhard Niessner2,
  3. Dietmar Knopp3

Published Online: 15 JUL 2006

DOI: 10.1002/14356007.b05_129.pub2

Ullmann's Encyclopedia of Industrial Chemistry

Ullmann's Encyclopedia of Industrial Chemistry

How to Cite

Miller, J. N., Niessner, R. and Knopp, D. 2006. Enzyme and Immunoassays. Ullmann's Encyclopedia of Industrial Chemistry. .

Author Information

  1. 1

    Department of Chemistry, Loughborough University, United Kingdom

  2. 2

    Technische Universität München, München, Germany

  3. 3

    Technische Universität München, München, Germany

Publication History

  1. Published Online: 15 JUL 2006

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The article contains sections titled:

1.Enzymatic Analysis Methods
1.2.Enzymes: Basic Kinetics
1.3.Enzyme Assays: Practical Aspects
1.3.1.General Considerations
1.3.2.Spectrometric Methods
1.3.3.Other Methods
1.3.4.Effects of pH, Buffer, Composition, and Temperature
1.3.5.Sources and Activity of Enzymes
1.4.Enzyme Assays: Determination of Substrates
1.4.1.General Considerations
1.4.2.Determination of Glucose
1.4.3.Determination of Ethanol
1.5.Enzyme Assays: Determination of Inhibitors
1.5.1.Inhibitors of Cholinesterase Enzymes
1.5.2.Other Inhibition Methods
1.6.Enzyme Assays: Determination of Activators
1.6.1.Determination of Metal Ions by Metalloenzymes
1.6.2.Other Activator Analyses
1.7.Immobilized Enzymes
1.7.2.Properties of Immobilized Enzymes
1.7.3.Application of Immobilized Enzymes
2.Immunoassays in Analytical Chemistry
2.2.Polyclonal, Monoclonal, and Recombinant Antibodies
2.3.Sample Conditioning
2.4.1.Radioimmunoassay Dilution Radioimmunoassay Assay (IRMA)
2.4.2.Nonisotopic Homogeneous Immunoassay Particle Agglutination Immunoassay Immunoassay Technique (EMIT) Reconstitution Immunoassay System (ARIS) Homogeneous Immunoassay Fluorescence Polarization Immunoassay (FPIA) Liposome Immunoassay
2.4.3.Nonisotopic Heterogeneous Immunoassay Immunosorbent Assay (ELISA) Immunoassay using Immobilized Affinity Ligands with Biotin and Avidin Particle Immunoassay
2.5.Immunoaffinity Techniques
2.6.Immunoassays on Test Strips and other Planar Structures
2.7.Characterization and Interference
2.8.Future Immunological Applications and Techniques