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Liquid Chromatography, 1. Fundamentals, History, Instrumentation, Materials

  1. Pavel Jandera1,
  2. Günter Henze2

Published Online: 15 OCT 2010

DOI: 10.1002/14356007.b05_237.pub2

Ullmann's Encyclopedia of Industrial Chemistry

Ullmann's Encyclopedia of Industrial Chemistry

How to Cite

Jandera, P. and Henze, G. 2010. Liquid Chromatography, 1. Fundamentals, History, Instrumentation, Materials. Ullmann's Encyclopedia of Industrial Chemistry. .

Author Information

  1. 1

    University of Pardubice, Pardubice, Czech Republic

  2. 2

    Institut für Anorganische und Analytische Chemie deA Technischen Universität, Clausthal, Germany

Publication History

  1. Published Online: 15 OCT 2010

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Abstract

The article contains sections titled:

1.Introduction
1.1.History
1.2.Definition and Theoretical Background
2.Equipment
2.1.Solvent Reservoir, Filters, Degasser and Connecting Tubing
2.2.Pumps
2.3.Sample Introduction Units
2.4.Column Hardware
2.5.Detectors
2.5.1.Photometric Detectors
2.5.2.The Fluorescence Detector
2.5.3.Refractive Index (RI) Detectors
2.5.4.Light-Scattering Detectors
2.5.5.Electrochemical Detectors
2.6.Data Processing Systems
3.Packing Materials and Stationary Phases for HPLC
3.1.Types and Porosity of Column Packing Materials
3.2.Particle Size, Hydrodynamic Aspects of Column Operation
3.3.Superficially Porous Particles
3.4.Monolithic Columns
3.5.Stationary Phases for Liquid Chromatography
3.5.1.Silica Gel
3.5.2.Metal Oxides
3.5.3.Chemically Bonded Phases
3.5.4.Carbon Adsorbents
3.5.5.Organic Polymer Adsorbents
3.5.6.Characterization of HPLC Column Properties
4.The Mobile Phase
4.1.Purification and Pretreatment of LC Solvents
4.2.Selection of Mobile Phase