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Biotechnology, 2. Metabolic Engineering

  1. Horst Werner Doelle1,
  2. Armin Fiechter2,
  3. Günther Schlegel3,
  4. Sakayu Shimizu4,
  5. Joachim Wegener5,
  6. Hideaki Yamada4

Published Online: 15 JUL 2009

DOI: 10.1002/14356007.n04_n02

Ullmann's Encyclopedia of Industrial Chemistry

Ullmann's Encyclopedia of Industrial Chemistry

How to Cite

Doelle, H. W., Fiechter, A., Schlegel, G., Shimizu, S., Wegener, J. and Yamada, H. 2009. Biotechnology, 2. Metabolic Engineering. Ullmann's Encyclopedia of Industrial Chemistry. .

Author Information

  1. 1

    University of Queensland, Department of Microbiology, St. Lucia, Queensland, Australia

  2. 2

    Eidgenössische Technische Hochschule, Institute of Biotechnology, Zürich, Switzerland

  3. 3

    University of Göttingen, Institute of Microbiology, Göttingen, Germany

  4. 4

    Kyoto University, Department of Agricultural Chemistry, Kyoto, Japan

  5. 5

    University of Münster, Institute of Biochemistry, Germany

Publication History

  1. Published Online: 15 JUL 2009

Abstract

The article contains sections titled:

1.Introduction
2.Analysis of the Transcriptome, Proteome, and Metabolome
2.1.Gene Expression Analysis using DNA Microarrays
2.2.Fabrication of DNA microarrays
2.3.Proteome Analysis using Protein Microarrays
2.4.Metabolome Analysis and Metabolite flux analysis
3.Design and Production of Genetically Optimized Strains for Production – In Vitro Mutagenesis
4.Random Mutagenesis, Isolation and Selection of Mutants
5.Types of Mutants and Selection Principles
5.1.Auxotrophic Mutants
5.2.Regulatory Mutants
5.3.Other Selection Methods
5.4.Targeted or Site-Directed Mutagenesis