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Liquid Chromatography, 2. Methods, Programmed and Coupling Techniques

  1. Pavel Jandera1,
  2. Peter Lembke2

Published Online: 15 OCT 2010

DOI: 10.1002/14356007.o15_o01

Ullmann's Encyclopedia of Industrial Chemistry

Ullmann's Encyclopedia of Industrial Chemistry

How to Cite

Jandera, P. and Lembke, P. 2010. Liquid Chromatography, 2. Methods, Programmed and Coupling Techniques. Ullmann's Encyclopedia of Industrial Chemistry. .

Author Information

  1. 1

    University of Pardubice, Pardubice, Czech Republic

  2. 2

    Belovo-Spain, S.L., Reus, Spain

Publication History

  1. Published Online: 15 OCT 2010

Chemistry Terms

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Abstract

The article contains sections titled:

1.Separation Processes and HPLC Modes
1.1.Normal-Phase Liquid Chromatography (NPLC)
1.1.1.Separations on Polar Adsorbents and Chemically Bonded Phases in Nonaqueous Mobile Phases
1.1.1.1.Principles of Retention in NPLC
1.1.1.2.Structural and Mobile Phase Effects in Nonaqueous NPLC
1.1.1.3.Practical Aspects and Application Examples of Nonaqueous NPLC
1.1.2.Normal-Phase Separations of Hydrophilic and Ionic Compounds in Aqueous-Organic Media - HILIC Mode
1.2.Reversed-Phase Liquid Chromatography (RPLC)
1.2.1.Separations of Nonionic Samples
1.2.2.Separations of Ionizable Samples with Buffer and Salt Additives
1.2.3.Ion-Pair Chromatography
1.2.4.Micellar Chromatography
1.3.Ion-Exchange Chromatography (IEX)
1.4.Size-Exclusion Chromatography (SEC)
2.Optimization of Separation Selectivity and Resolution
2.1.Optimization of the Column Parameters
2.2.Two-Component (Binary) Mobile Phases
2.3.Selectivity Control using Ternary or more Complex Mobile Phases
2.4.Computer-Assisted HPLC Method Development and Optimization
3.Programmed and Coupling Techniques
3.1.Gradient-Elution Technique
3.2.High-Temperature and Programmed Temperature HPLC Separations
3.3.Column Serial Coupling and Switching
3.4.Multidimensional HPLC
3.4.1.Peak Capacity and 2D System Orthogonality
3.4.2.Off-Line and In-Line Heart Cutting Techniques
3.4.3.Comprehensive Two-Dimensional LCxLC
3.4.4.Applications of 2D LC-LC
3.5.HPLC– Mass Spectrometry
3.5.1.Principles of the HPLC-MS Technique
3.5.2.Instrumentation for HPLC-MS
3.5.3.Applications of LC-MS
3.6.HPLC-NMR
4.Quantitative Analysis
4.1External Standard Method
4.2.Internal Standard Method
4.3.Error Sources in HPLC
5.Sample Preparation and Derivatization
5.1.Sample Cleanup and Enrichment
5.2.Pre- and Postcolumn Derivatization
5.2.1.Precolumn Derivatization
5.2.2.Postcolumn Derivatization