Communication

Rubbed Films of Functionalized Bovine Serum Albumin as Substrates for the Imaging of Protein–Receptor Interactions Using Liquid Crystals

  1. S.-R. Kim,
  2. N. L. Abbott

Article first published online: 27 SEP 2001

DOI: 10.1002/1521-4095(200110)13:19<1445::AID-ADMA1445>3.0.CO;2-9

Issue

Advanced Materials

Advanced Materials

Volume 13, Issue 19, pages 1445–1449, October, 2001

Additional Information

How to Cite

Kim, S.-R. and Abbott, N. L. (2001), Rubbed Films of Functionalized Bovine Serum Albumin as Substrates for the Imaging of Protein–Receptor Interactions Using Liquid Crystals. Adv. Mater., 13: 1445–1449. doi: 10.1002/1521-4095(200110)13:19<1445::AID-ADMA1445>3.0.CO;2-9

Author Information

  1. Department of Chemical Engineering, University of Wisconsin, Madison, 1415 Engineering Drive, Madison, WI 53705, USA

Publication History

  1. Issue published online: 27 SEP 2001
  2. Article first published online: 27 SEP 2001
  3. Manuscript Accepted: 23 MAY 2001
  4. Manuscript Received: 12 MAR 2001

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Keywords:

  • Bovine serum albumin (BSA);
  • Ellipsometry;
  • Immunglobulin G (IgG), anti-biotin;
  • Liquid crystals (LC), nematic;
  • Protein films

Rubbing is an essential pre-treatment for covalently immobilized, receptor-functionalized films of bovine serum albumin to function in liquid-crystal (LC) imaged protein chips. Only on the rubbed film will LCs form a nematic phase whose disruption by docking target protein is specific and quantifiable using polarized light. The Figure shows the appearance at two anti-biotin IgG concentrations, the polarizer aligned with the direction of rubbing (left) and shifted by 45° (right).

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