• 1
    Sobotka, A. K., Valentine, M. D., Ishizaka, K. and Lichtenstein, L. M., Measurement of IgG-blocking antibodies: development and application of a radioimmunoassay. J. Immunol. 1976. 117: 8490.
  • 2
    Golden, D. B., Meyers, D. A., Kagey Sobotka, A., Valentine, M. D. and Lichtenstein, L. M., Clinical relevance of the venom-specific immunoglobulin G antibody level during immunotherapy. J. Allergy Clin. Immunol. 1982. 69: 489493.
  • 3
    Aalberse, R. C., van der Gaag, R. and van Leeuwen, J., Serologic aspects of IgG4 antibodies. I. Prolonged immunization results in an IgG4–restricted response. J. Immunol. 1983. 130: 722726.
  • 4
    McHugh, S.M., Lavelle, B., Kemeny, D. M., Patel, S. and Ewan,P. W., A placebo-controlled trial of immunotherapy with two extracts of Dermatophagoides pteronyssinus in allergic rhinitis, comparing clinical outcome with changes inantigen-specific IgE, IgG, and IgG subclasses. J. Allergy Clin. Immunol. 1990. 86: 521531.
  • 5
    Ewan, P. W., Deighton, J., Wilson, A. B. and Lachmann, P. J., Venom-specific IgG antibodies in bee and wasp allergy: lack of correlation with protection from stings. Clin. Exp. Allergy 1993. 23: 647660.
  • 6
    Wilson, A. B., Deighton, J., Lachmann, P. J. and Ewan, P. W., A comparative study of IgG subclass antibodies in patients allergic to wasp or bee venom. Allergy. 1994. 49: 272280.
  • 7
    Blaauw, P. J. and Smithuis, L. O., The evaluation of the common diagnostic methods of hypersensitivity for bee and yellow jacket venom by means of an in-hospital insect sting. J. Allergy Clin. Immunol. 1985. 75: 556562.
  • 8
    Kampelmacher, M. J. and van der Zwan, J. C., Provocation test with a living insect as a diagnostic tool in systemic reactions to bee and wasp venom: a prospective study with emphasis on the clinical aspects. Clin. Allergy 1987. 17: 317327.
  • 9
    Muller, U., Helbling, A. and Bischof, M., Predictive value of venom-specific IgE, IgG and IgG subclass antibodies in patients on immunotherapy with honey bee venom. Allergy. 1989. 44: 412418.
  • 10
    Del Prete, G., Maggi, E., Parronchi, P., Chretien, I., Tiri,A., Macchia, D., Ricci, M., Banchereau, J., De Vries, J. and Romagnani, S., IL–4 is an essential factor for the IgE synthesis induced in vitro by human T cell clones and their supernatants. J. Immunol. 1988.140: 41934198.
  • 11
    Punnonen, J., Aversa, G., Cocks, B. G., McKenzie, A.N., Menon, S., Zurawski, G., de Waal Malefyt, R. and de Vries, J. E., Interleukin 13 induces interleukin 4-independent IgG4 and IgE synthesis and CD23 expression by human B cells. Proc. Natl. Acad. Sci. USA 1993. 90: 37303734.
  • 12
    Walker, C., Virchow, J. C., Jr., Bruijnzeel, P. L. and Blaser, K., T cell subsets and their soluble products regulate eosinophilia in allergic and nonallergic asthma. J. Immunol. 1991. 146: 18291835.
  • 13
    Jutel, M., Pichler, W. J., Skrbic, D., Urwyler, A., Dahinden, C. and Muller, U. R., Bee venom immunotherapy results in decrease of IL–4 and IL–5 and increase of IFN–gamma secretion in specific allergen-stimulated T cell cultures. J. Immunol. 1995. 154: 41874194.
  • 14
    McHugh, S. M., Deighton, J., Stewart, A. G., Lachmann, P. J. and Ewan, P. W., Bee venom immunotherapy induces a shift in cytokine responses from a TH-2 to a TH-1 dominant pattern: comparison of rush and conventional immunotherapy. Clin. Exp. Allergy 1995. 25: 828838.
  • 15
    Rocklin, R. E., Immune mechanisms in allergen-specific immunotherapy. Clin. Immunol. Immunopathol. 1989. 53: S119–S131.
  • 16
    Fling, J. A., Ruff, M. E., Parker, W. A., Whisman, B. A., Martin, M. E., Moss, R. B. and Reid, M. J., Suppression of the late cutaneous response by immunotherapy. J. Allergy Clin. Immunol. 1989. 83: 101109.
  • 17
    Varney, V. A., Hamid, Q. A., Gaga, M., Ying, S., Jacobson, M., Frew, A. J., Kay, A. B. and Durham, S. R., Influence of grass pollen immunotherapy on cellular infiltration and cytokine mRNA expression during allergen-induced late-phase cutaneous responses. J. Clin. Invest. 1993. 92: 644651.
  • 18
    Warner, J. O., Price, J. F., Soothill, J. F. and Hey, E. N., Controlled trial of hyposensitisation to Dermatophagoides pteronyssinus in children with asthma. Lancet 1978. 2: 912915.
  • 19
    Bousquet, J., Calvayrac, P., Guerin, B., Hejjaoui, A., Dhivert, H., Hewitt, B. and Michel, F. B., Immunotherapy with a standardized Dermatophagoides pteronyssinus extract. I. In vivo and in vitro parameters after a short course of treatment. J. Allergy Clin. Immunol. 1985. 76: 734744.
  • 20
    Van Bever, H. P. and Stevens, W. J., Suppression of the late asthmatic reaction by hyposensitization in asthmatic children allergic to house dust mite (Dermatophagoides pteronyssinus). Clin. Exp. Allergy 1989. 19: 399404.
  • 21
    Gesser, B., Deleuran, B., Lund, M., Vestergard, C., Lohse, N., Deleuran, M., Jensen, S. L., Pedersen, S. S., Thestrup-Pedersen, K. and Larsen, C. G. Interleukin-8 induces its own production in CD4+ T lyumphocytes: a process regulated by interleukin-10. Biochem. Biophys. Res. Commun .1995. 210:660669
  • 22
    Wang, P., Wu, P., Anthes, J. C., Siegel, M. I., Egtan, R. W. and Billah, M. M. Interleukin-10 inhibits interleukin-8 production in human neutrophils. Blood 1994. 83:26782683
  • 23
    Fiorentino, D. F., Bond, M. W. and Mosmann, T. R., Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones. J. Exp. Med. 1989. 170: 20812095.
  • 24
    de Waal Malefyt, R., Haanen, J., Spits, H., Roncarolo, M. G., te Velde, A., Figdor, C., Johnson, K., Kastelein, R., Yssel, H. and de Vries, J. E., Interleukin 10 (IL–10) and viral IL–10 strongly reduce antigen-specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression. J. Exp. Med. 1991. 174: 915924.
  • 25
    de Waal Malefyt, R., Abrams, J., Bennett, B., Figdor, C. G. and de Vries, J. E., Interleukin 10 inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL–10 produced by monocytes. J. Exp. Med. 1991. 174: 12091220.
  • 26
    Zuany Amorim, C., Haile, S., Leduc, D., Dumarey, C., Huerre,M., Vargaftig, B. B. and Pretolani, M., Interleukin–10 inhibits antigen-induced cellular recruitment into the airways ofsensitized mice. J. Clin. Invest. 1995. 95: 26442651.
  • 27
    Zuany Amorim, C., Creminon, C., Nevers, M. C., Nahori, M. A., Vargaftig, B. B. and Pretolani, M., Modulation by IL–10 of antigen-induced IL–5 generation, and CD4+ T lymphocyte and eosinophil infiltration into the mouse peritoneal cavity. J. Immunol. 1996. 157: 377384.
  • 28
    Schandene, L., Alonso Vega, C., Willems, F., Gerard, C., Delvaux, A., Velu, T., Devos, R., de Boer, M. and Goldman, M., B7/CD28-dependent IL–5 production by human resting T cells is inhibited by IL–10. J. Immunol. 1994. 152: 43684374.
  • 29
    de Waal Malefyt, R., Yssel, H. and de Vries, J. E., Direct effects of IL–10 on subsets of humanCD4+ T cell clones and resting T cells. Specific inhibition of IL–2 production and proliferation. J. Immunol. 1993. 150: 47544765.
  • 30
    Takanaski, S., Nonaka, R., Xing, Z., O'Byrne, P., Dolovich, J. and Jordana, M., Interleukin 10 inhibits lipopolysaccharide-induced survival and cytokine production by human peripheral blood eosinophils. J. Exp. Med. 1994. 180: 711715.
  • 31
    Ohkawara, Y., Lim, K. G., Xing, Z., Glibetic, M., Nakano, K., Dolovich, J., Croitoru, K., Weller, P. F. and Jordana, M., CD40expression by human peripheral blood eosinophils. J. Clin. Invest. 1996. 97: 17611766.
  • 32
    Enk, A. H., Saloga, J., Becker, D., Mohamadzadeh, M. and Knop, J., Induction of hapten-specific tolerance by interleukin 10 in vivo. J. Exp. Med. 1994. 179: 13971402.
  • 33
    Stampfli, M. R., Cwiartka, M., Gajewska, B. U., Alvarez, D., Ritz, S. A., Inman, M. D., Xing, Z. and Jordana, M., Interleukin–10 gene transfer to the airway regulates allergic mucosal sensitization in mice. Am. J. Respir. Cell. Mol. Biol. 1999. 21: 586596.
  • 34
    Ying, S., Meng, Q., Barata, L. T., Robinson, D. S., Durham, S. R. and Kay, A. B., Associations between IL–13 and IL–4 (mRNA and protein), vascular cell adhesion molecule-1 expression, and the infiltration of eosinophils, macrophages, and T cells in allergen-induced late-phase cutaneous reactions in atopic subjects. J. Immunol. 1997. 158: 50505057.
  • 35
    Barata, L. T., Ying, S., Meng, Q., Barakans, J., Rajakulasingham, K. and Durham, S. R., IL–4 and IL–5-positive T lymphocytes, eosinophils and mast cells in allergen-induced late-phase cutaneous reactions in atopic subjects. J. Allergy Clin. Immunol. 1998. 101: 222230.
  • 36
    Akdis, C.A., Blesken, T., Akdis, M., Wuthrich, B. and Blaser, K., Role of interleukin 10 in specific immunotherapy. J. Clin Invest 1998. 102: 98106.
  • 37
    Akdis, C.A., Akdis, M., Blesken, T., Wymann, D., Alkan, S. S., Muller, U. and Blaser, K., Epitope-specific T cell tolerance to phospholipase A2 in bee venom immunotherapy and recovery by IL–2 and IL–15 in vitro. J. Clin. Inves.t 1996. 98: 16761683.
  • 38
    Durham, S. R., Ying, S., Varney, V. A., Jacobson, M. R., Sudderick, R. M., Mackay, I. S., Kay, A. B. and Hamid, Q. A., Grass pollen immunotherapy inhibits allergen-induced infiltration of CD4+ T lymphocytes and eosinophils in the nasal mucosa and increases the number of cells expressing messenger RNA for interferon–gamma. J. Allergy Clin. Immunol. 1996. 97: 13561365.
  • 39
    Durham, S. R., Walker, S. M., Varga, E. M., Jacobson, M. R., O'Brien, F., Noble, W., Till, S. J., Hamid, Q. A. and Nouri Aria, K. T., Long-term clinical efficacy of grass-pollen immunotherapy N. Engl. J. Med. 1999. 341: 468475.
  • 40
    Fiorentino, D. F., Zlotnik, A., Vieira, P., Mosmann, T. R., Howard, M., Moore, K. W. and O'Garra, A., IL–10 acts on the antigen-presenting cell to inhibit cytokine production by Th1 cells. J. Immunol. 1991. 146: 34443451.
  • 41
    Bellinghausen, I., Metz, G., Enk, A. H., Christmann, S., Knop, J. and Saloga, J., Insect venom immunotherapy induces interleukin–10 production and a Th2 to Th1 shift, and changes surface marker expression in venom-allergic subjects. Eur. J. Immunol. 1997. 27: 11311139.
  • 42
    Borish, L., Aarons, A., Rumbyrt, J., Cvietusa, P., Negri, J. and Wenzel, S., Interleukin–10 regulation in normal subjects and patients with asthma. J. Allergy Clin. Immunol. 1996. 97: 12881296.
  • 43
    Britten, K. M., Howarth, P. H. and Roche, W. R., Immunohistochemistry on resin sections: a comparison of resin embedding techniques for small mucosal biopsies. Biotech. Histochem. 1993. 68: 271280.
  • 44
    Frew, A. J. and Kay, A. B., The relationship between infiltrating CD4+ lymphocytes, activated eosinophils, and themagnitude of the allergen-induced late phase cutaneous reaction in man. J. Immunol. 1988. 141: 41584164.
  • 45
    Gaga, M., Frew, A. J., Varney, V. A. and Kay, A. B., Eosinophil activation and T lymphocyte infiltration in allergen-induced late phase skin reactions and classical delayed-type hypersensitivity. J. Immunol. 1991. 147: 816822.
  • 46
    Ying, S., Robinson, D. S., Meng, Q., Barata, L. T., McEuen, A. R., Buckley, M. G., Walls, A. F., Askenase, P. W. and Kay, A. B., C-C chemokines in allergen-induced late-phase cutaneous responses in atopic subjects: association of eotaxin with early 6-hour eosinophils, and of eotaxin-2 and monocyte chemoattractant protein-4 with the later 24-hour tissue eosinophilia, and relationship to basophils and other C-C chemokines (monocyte chemoattractant protein-3 and RANTES). J. Immunol. 1999. 163: 39763984.