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Full Paper
Accuracy of 1H and 31P MRS analyses of lactate in skeletal muscle
Article first published online: 1 SEP 2000
DOI: 10.1002/1522-2594(200009)44:3<418::AID-MRM12>3.0.CO;2-G
Copyright © 2000 Wiley-Liss, Inc.
1522-2594/asset/cover.gif?v=1&s=9512352d4133369aa19bc75e4ffa8001ea23b2d9 "Magnetic Resonance in Medicine")
Additional Information
How to Cite
Hsu, A. C. and Dawson, M. J. (2000), Accuracy of 1H and 31P MRS analyses of lactate in skeletal muscle. Magnetic Resonance in Medicine, 44: 418–426. doi: 10.1002/1522-2594(200009)44:3<418::AID-MRM12>3.0.CO;2-G
Publication History
- Issue published online: 1 SEP 2000
- Article first published online: 1 SEP 2000
- Manuscript Accepted: 5 MAY 2000
- Manuscript Revised: 4 MAY 2000
- Manuscript Received: 27 SEP 1999
Funded by
- PHS. Grant Number: 5 P41 RR05964
- NIH. Grant Number: CA09067
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Keywords:
- lactate;
- buffer capacity;
- muscle;
- 1H NMR;
- 31P NMR;
- MRS
Abstract
As the end product of anaerobic metabolism and a source of H+, lactic acid is important in metabolism and pH regulation. Several methods have been introduced to calculate changes in the lactate anion (Lac–) concentration in exercising skeletal muscle from information derived from the 31P spectrum. Alternatively, Lac–may be observed directly with 1H MRS. Both 1H and 31P spectroscopy have potential problems, which could prevent accurate determination of [Lac–]. It is demonstrated that quantitatively accurate 1H MRS measurements of changes in [Lac–] due to exercise are possible in isolated muscle. In general, calculation by 31P MRS overestimates Lac–production. An analysis is presented of possible sources of errors in the 1H and 31P MRS methods. Magn Reson Med 44:418–426, 2000. © 2000 Wiley-Liss, Inc.