This article is a US Government work and is in the public domain in the USA.
Phytotoxicity of selected trichothecenes using Chlamydomonas reinhardtii as a model system †
Article first published online: 13 DEC 2000
This article is a US government work and is in the public domain in the United States.
Volume 7, Issue 6, pages 265–269, November/December 1999
How to Cite
Alexander, N. J., McCormick, S. P. and Ziegenhorn, S. L. (1999), Phytotoxicity of selected trichothecenes using Chlamydomonas reinhardtii as a model system . Nat. Toxins, 7: 265–269. doi: 10.1002/1522-7189(199911/12)7:6<265::AID-NT65>3.0.CO;2-5
- Issue published online: 13 DEC 2000
- Article first published online: 13 DEC 2000
- Manuscript Accepted: 22 FEB 2000
- Manuscript Received: 4 FEB 2000
- Fusarium toxins;
- wheat head blight;
Trichothecenes are potent inhibitors of cytoplasmic protein synthesis which can affect the severity of plant diseases such as wheat head scab. While many trichothecene-producing fungi share the initial biosynthetic intermediates, Fusarium sp. are unique in the production of trichothecenes containing an oxygen function at C-3. Although the initial trichothecene and the final products have a C-3 hydroxyl group, the intermediate steps are acetylated at C-3. By using Chlamydomonas reinhardtii, a unicellular plant with a well-defined genetic system, we were able to test the proposal that trichothecenes with a C-3 hydroxyl are more toxic to plants, as well as demonstrate that C. reinhardtii is a promising plant trichothecene bioassay system. Seven pairs of trichothecenes with either a C-3 hydroxyl or C-3 acetyl group were assayed. Our results confirm that trichothecenes acetylated at C-3 were far less toxic to Chlamydomonas than those with a C-3 hydroxyl group. Published in 1999 by John Wiley & Sons, Ltd.