Immunohistochemistry of fumonisin in poultry using avidin–biotin–peroxidase system

Authors

  • Marcos Roberto Buim,

    1. Department of Food and Drug Technology, and Department of Preventive Veterinary Medicine, Center of Agricultural Sciences, State University of Londrina, Londrina, Paraná, P.O. Box 6001, 86051-990, Brazil.
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  • Ana Paula Frederico Rodrigues Loureiro Bracarense,

    1. Department of Food and Drug Technology, and Department of Preventive Veterinary Medicine, Center of Agricultural Sciences, State University of Londrina, Londrina, Paraná, P.O. Box 6001, 86051-990, Brazil.
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  • Ivens Gomes Guimarães,

    1. Department of Food and Drug Technology, and Department of Preventive Veterinary Medicine, Center of Agricultural Sciences, State University of Londrina, Londrina, Paraná, P.O. Box 6001, 86051-990, Brazil.
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  • Osamu Kawamura,

    1. Faculty of Pharmaceutical Science, Science University of Tokyo, 12 Ichigaya, Funagawara-Machi, Tokyo 162-0826
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  • Yoshio Ueno,

    1. Department of Cellular Toxicology, Tochigi Institute of Clinical Pathology, 2308-3 Minami-Akatsuka, Simotsuga-gun, Tochigi 329-01112, Japan
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  • Elisa Yoko Hirooka

    Corresponding author
    1. Department of Food and Drug Technology, and Department of Preventive Veterinary Medicine, Center of Agricultural Sciences, State University of Londrina, Londrina, Paraná, P.O. Box 6001, 86051-990, Brazil.
    • Department of Food and Drug Technology, Center of Agricultural Sciences, State University of Londrina, Londrina PR, P.O. Box 6001, CEP 86051-990, Brazil
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Abstract

Using monoclonal anti-fumonisin B1 antibody (anti-FB1) and avidin–biotin–peroxidase system, liver and kidneys of broiler chicks were evaluated for the detection and distribution of fumonisins (FBs). One hundred and fifty micrograms of FB1 or culture extract of Fusarium moniliforme str. 113F containing 150 µg of FB1 and 4 µg of FB2 were administered into the vitelline sac of 1-day old, specific pathogen-free chicks. The animals were killed 24 h after injection, and renal and hepatic tissues submitted for immunohistochemical analysis. FBs were detected in the epithelial cells of convoluted distal and proximal tubules of the kidneys, as well as in the cytoplasm of hepatocytes. This novel immunohistochemical method developed is expected to be an efficient way for monitoring the target of the FB toxins in tissues. Copyright © 1999 John Wiley & Sons, Ltd.

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