Detection of diarrhetic shellfish poisoning toxins from tropical shellfish using liquid chromatography-selected reaction monitoring mass spectrometry

Authors

  • Michael J. Holmes,

    Corresponding author
    1. Department of Biological Sciences and National University of Singapore, 119260 Singapore
    2. Tropical Marine Science Institute, National University of Singapore, 119260 Singapore
    • Department of Biological Sciences, National University of Singapore, Singapore, 119260
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  • Serena Lay Ming Teo,

    1. Tropical Marine Science Institute, National University of Singapore, 119260 Singapore
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  • Hong Woo Khoo

    1. Department of Biological Sciences and National University of Singapore, 119260 Singapore
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Abstract

A negative mode liquid chromatography-selected reaction monitoring mass spectrometry (LC-SRM MS) method was developed to detect low concentrations of the diarrhetic shellfish poisoning (DSP) toxins okadaic acid and dinophysistoxin-1 (DTX-1). Detection relies upon monitoring the transition of negative precursor ions [M − H] to a common fragment ion of m/z 255. Our limit of detection for okadaic acid with this method is 0.5 pg on column. LC-SRM MS has allowed us to detect persistent, low concentrations of DSP toxins from Singapore shellfish. Copyright © 1999 John Wiley & Sons, Ltd.

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