Toxigenic Corynebacterium ulcerans in human and non-toxigenic Corynebacterium diphtheriae in cat

Authors

  • L. Detemmerman,

    1. National Reference Centre for Corynebacterium diphtheriae, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium
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  • D. Rousseaux,

    1. Laboratoire de Biologie Clinique, Clinique Sainte-Elisabeth, Heusy, Belgium
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  • A. Efstratiou,

    1. WHO Global Collaborating Centre for Diphtheria & Streptococcal Infections, Public Health England, London, United Kingdom
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  • C. Schirvel,

    1. Cellule d'Inspection d'Hygiène, Direction générale de la Santé, Fédération Wallonie-Bruxelles, Brussels, Belgium
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  • K. Emmerechts,

    1. National Reference Centre for Corynebacterium diphtheriae, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium
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  • I. Wybo,

    1. National Reference Centre for Corynebacterium diphtheriae, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium
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  • O. Soetens,

    1. National Reference Centre for Corynebacterium diphtheriae, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium
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  • D. Piérard

    Corresponding author
    1. National Reference Centre for Corynebacterium diphtheriae, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium
    • Corresponding author: D. Piérard, National Reference Centre for Corynebacterium diphtheriae, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Laarbeeklaan 101, Brussels, Belgium. E-mail: Denis.Pierard@uzbrussel.be

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Abstract

Corynebacterium diphtheriae and Corynebacterium ulcerans are rarely isolated from clinical samples in Belgium. A case of toxigenic C. ulcerans in a woman is described, which confirms that this pathogen is still present. During investigation of the patient's cats, only a non-toxigenic toxin-bearing C. diphtheriae strain was detected.

Case Report

In May 2012, a 72-year-old woman presented with a chronic leg ulceration from which Corynebacterium ulcerans was isolated in mixed culture with Enterobacter aerogenes, Staphylococcus aureus and Streptococcus agalactiae. No systemic symptoms were recorded. A booster with Tedivax pro adulto® was documented in 2007. She had no history of recent travel but was living in a trailer in poor hygienic conditions, with several cats. The condition of the patient improved quickly with oral amoxicillin.

The identification was confirmed as C. ulcerans by the National Reference Centre for Corynebacterium diphtheriae. The toxin gene was detected by conventional PCR [1]. A 900-base-pair (bp) PCR fragment of the toxin gene of C. ulcerans showed 100% identity with AB610405 and FJ858272 toxin gene sequences of C. ulcerans strains and 97% or less similarity with sequences from C. diphtheriae strains. As PCR is not an indicator of toxin expression, the strain was sent to the WHO Global Collaborating Centre for Diphtheria (Public Health England, London) for confirmation of toxigenicity by the Elek immunoprecipitation test.

All cultures from close contacts were negative. As C. ulcerans, a zoonotic pathogen [2-4], could be transmitted from animals, an attempt was made to screen the patient's four cats but only two could be captured. Corynebacterium diphtheriae biovar mitis was cultured from one. The toxin gene was detected but Elek testing was negative. Sequencing the complete toxin gene showed 99% sequence similarity to other C. diphtheriae toxin gene sequences but a 1-bp deletion at position 55 resulted in a prematurely terminated peptide at amino acid 38. Multi-locus sequence typing [5] of this strain resulted in ST40 (Table 1).

Table 1. Characteristics of the isolates
StrainSourceIdentificationToxin PCR resultElek resultToxin gene sequence GenBank ref.Multilocus sequence typing result
DIFT019Human skin lesion Corynebacterium ulcerans ++

889 base pairs

KF013949

Not available
DIFT020Nose swab from catCorynebacterium diphtheriae biovar mitis+

1682 base pairs

KF013950

ST40

Since the outbreak in eastern Europe in the 1990s, only rare cases of C. diphtheriae have been observed in Europe [6]. The genus Corynebacterium comprises three species with toxigenic potential: C. diphtheriae, C. ulcerans and Corynebacterium pseudotuberculosis. Corynebacterium ulcerans has a broad host range and humans and other mammals can be infected [2, 4]. Our patient was probably protected by her recent vaccine booster, as she did not present systemic symptoms. Although zoonotic transmission could not be proven here, this remains the most probable source of infection [2, 3].

Curiously, a non-toxigenic C. diphtheriae strain was isolated from a cat, suggesting that humans might not be the sole reservoir. Such non-toxigenic feline isolates have already been reported [7], all presenting a 1-bp deletion at nucleotide 55 in the toxin gene, in spite of only 95% sequence similarity. This suggests that closely related toxin genes are present in the feline isolates and questions the role of cats as reservoirs for human infections.

This case report shows that immunization against diphtheria toxin remains important not only because of the threat of cases imported from other parts of the world but also because of the zoonotic potential of toxigenic C. ulcerans strains in Europe.

Acknowledgements

The authors want to express their gratitude to all the persons who contributed to the management of this case, in particular Anne Makaire who sampled the cats and Josiane Wislez and Thierry Willems who treated the patient in the revalidation centre. The sequenced toxin gene fragments of the C. ulcerans and C. diphtheriae were submitted to GenBank under reference numbers KF013949 and KF013950, respectively.

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