Chapter 2. Expression Proteomics
Published Online: 7 MAY 2002
DOI: 10.1002/3527600175.ch2
Copyright © 2002 Wiley-VCH Verlag GmbH
Book Title
Proteomics in Practice: A Laboratory Manual of Proteome Analysis
Additional Information
How to Cite
Westermeier, R. and Naven, T. (2002) Expression Proteomics, in Proteomics in Practice: A Laboratory Manual of Proteome Analysis, Wiley-VCH Verlag GmbH, Weinheim, FRG. doi: 10.1002/3527600175.ch2
Publication History
- Published Online: 7 MAY 2002
- Published Print: 9 MAY 2002
ISBN Information
Print ISBN: 9783527303540
Online ISBN: 9783527600175
- Summary
- Chapter
Keywords:
- proteomics technology;
- two-dimensional electrophoresis;
- sample preparation;
- isoelectric focusing;
- SDS-PAGE;
- protein spots;
- image analysis;
- spot handling;
- spot cutting;
- protein cleavage;
- mass spectrometry;
- tandem mass spectrometry (MS/MS);
- protein mass fingerprint;
- peptide mass fingerprint;
- de novo sequencing;
- 2D-MS;
- LC-MS/MS;
- quantitative proteomics
Summary
Two-dimensional Electrophoresis
Evolution of 2-D methodology
Sample preparation
First dimension: Isoelectric focusing
Second dimension: SDS-PAGE
Detection of protein spots
Image analysis
Spot handling
Spot cutting
Protein cleavage
Mass spectrometry
Ionisation
Ion separation
Tandem mass spectrometry (MS/MS)
Protein identification by database searching
Protein mass fingerprint
Peptide mass fingerprinting combined with composition information
Peptide mass fingerprint combined with partial sequence information
Product ion MS/MS sequence data
De novo sequencing
Methods of proteome analysis
2D-MS
LC-MS/MS
Quantitative proteomics