Chapter 11. Advanced Techniques in Fluorescence Spectroscopy
Published Online: 20 DEC 2001
DOI: 10.1002/3527600248.ch11
Copyright © 2001 Wiley-VCH Verlag GmbH
Book Title
Molecular Fluorescence: Principles and Applications
Additional Information
How to Cite
Valeur, B. (2001) Advanced Techniques in Fluorescence Spectroscopy, in Molecular Fluorescence: Principles and Applications, Wiley-VCH Verlag GmbH, Weinheim, FRG. doi: 10.1002/3527600248.ch11
Publication History
- Published Online: 20 DEC 2001
- Published Print: 21 DEC 2001
ISBN Information
Print ISBN: 9783527299195
Online ISBN: 9783527600243
- Summary
- Chapter
Keywords:
- time-resolved fluorescence;
- fluorescence up-conversion technique;
- fluorescence microscopy;
- confocal fluorescence microscopy;
- two-photon excitation fluorescence microscopy;
- near-field scanning optical microscopy (NSOM);
- fluorescence lifetime imaging spectroscopy (FLIM);
- time-domain FLIM;
- frequency-domain FLIM;
- confocal FLIM (CFLIM);
- two-photon FLIM;
- fluorescence correlation spectroscopy;
- single-molecule fluorescence spectroscopy
Summary
Time-resolved fluorescence in the femtosecond time range: fluorescence up-conversion technique
Advanced fluorescence microscopy
Improvements in conventional fluorescence microscopy
Confocal fluorescence microscopy
Two-photon excitation fluorescence microscopy
Near-field scanning optical microscopy (NSOM)
Fluorescence lifetime imaging spectroscopy (FLIM)
Time-domain FLIM
Frequency-domain FLIM
Confocal FLIM (CFLIM)
Two-photon FLIM
Fluorescence correlation spectroscopy
Conceptual basis and instrumentation
Determination of translational diffusion coefficients
Chemical kinetic studies
Determination of rotational diffusion coefficients
Single-molecule fluorescence spectroscopy
General remarks
Single-molecule detection in flowing solutions
Single-molecule detection using advanced fluorescence microscopy techniques