Chapter 11. Advanced Techniques in Fluorescence Spectroscopy

  1. Prof. Dr. Bernard Valeur

Published Online: 20 DEC 2001

DOI: 10.1002/3527600248.ch11

Molecular Fluorescence: Principles and Applications

Molecular Fluorescence: Principles and Applications

How to Cite

Valeur, B. (2001) Advanced Techniques in Fluorescence Spectroscopy, in Molecular Fluorescence: Principles and Applications, Wiley-VCH Verlag GmbH, Weinheim, FRG. doi: 10.1002/3527600248.ch11

Author Information

  1. Laboratoire de Chimie Générale, Conservatoire National des Arts et Métiers, 292 rue Saint-Martin, 75141 Paris Cedex 03, France

Publication History

  1. Published Online: 20 DEC 2001
  2. Published Print: 21 DEC 2001

ISBN Information

Print ISBN: 9783527299195

Online ISBN: 9783527600243

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Keywords:

  • time-resolved fluorescence;
  • fluorescence up-conversion technique;
  • fluorescence microscopy;
  • confocal fluorescence microscopy;
  • two-photon excitation fluorescence microscopy;
  • near-field scanning optical microscopy (NSOM);
  • fluorescence lifetime imaging spectroscopy (FLIM);
  • time-domain FLIM;
  • frequency-domain FLIM;
  • confocal FLIM (CFLIM);
  • two-photon FLIM;
  • fluorescence correlation spectroscopy;
  • single-molecule fluorescence spectroscopy

Summary

  • Time-resolved fluorescence in the femtosecond time range: fluorescence up-conversion technique

  • Advanced fluorescence microscopy

    • Improvements in conventional fluorescence microscopy

      • Confocal fluorescence microscopy

      • Two-photon excitation fluorescence microscopy

      • Near-field scanning optical microscopy (NSOM)

    • Fluorescence lifetime imaging spectroscopy (FLIM)

      • Time-domain FLIM

      • Frequency-domain FLIM

      • Confocal FLIM (CFLIM)

      • Two-photon FLIM

  • Fluorescence correlation spectroscopy

    • Conceptual basis and instrumentation

    • Determination of translational diffusion coefficients

    • Chemical kinetic studies

    • Determination of rotational diffusion coefficients

  • Single-molecule fluorescence spectroscopy

    • General remarks

    • Single-molecule detection in flowing solutions

    • Single-molecule detection using advanced fluorescence microscopy techniques