Chapter 5. Fluorescence Polarization. Emission Anisotropy

  1. Prof. Dr. Bernard Valeur

Published Online: 20 DEC 2001

DOI: 10.1002/3527600248.ch5

Molecular Fluorescence: Principles and Applications

Molecular Fluorescence: Principles and Applications

How to Cite

Valeur, B. (2001) Fluorescence Polarization. Emission Anisotropy, in Molecular Fluorescence: Principles and Applications, Wiley-VCH Verlag GmbH, Weinheim, FRG. doi: 10.1002/3527600248.ch5

Author Information

  1. Laboratoire de Chimie Générale, Conservatoire National des Arts et Métiers, 292 rue Saint-Martin, 75141 Paris Cedex 03, France

Publication History

  1. Published Online: 20 DEC 2001
  2. Published Print: 21 DEC 2001

ISBN Information

Print ISBN: 9783527299195

Online ISBN: 9783527600243

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Keywords:

  • polarization state of fluorescence;
  • polarization ratio;
  • emission anisotropy;
  • steady-state anisotropy;
  • instantaneous anisotropy;
  • emission transition moments;
  • rotation

Summary

  • Characterization of the polarization state of fluorescence (polarization ratio, emission anisotropy)

    • Excitation by polarized light

      • Vertically polarized excitation

      • Horizontally polarized excitation

    • Excitation by natural light

  • Instantaneous and steady-state anisotropy

    • Instantaneous anisotropy

    • Steady-state anisotropy

  • Additivity law of anisotropy

  • Relation between emission anisotropy and angular distribution of the emission transition moments

  • Case of motionless molecules with random orientation

    • Parallel absorption and emission transition moments

    • Non-parallel absorption and emission transition moments

  • Effect of rotational Brownian motion

    • Free rotations

    • Hindered rotations

  • Applications