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Single Molecule Biophysics with Fluorescence

  1. Michael Prummer,
  2. Christian Huebner

Published Online: 15 AUG 2009

DOI: 10.1002/3527600434.eap676

Encyclopedia of Applied Physics

Encyclopedia of Applied Physics

How to Cite

Prummer, M. and Huebner, C. 2009. Single Molecule Biophysics with Fluorescence. Encyclopedia of Applied Physics. .

Publication History

  1. Published Online: 15 AUG 2009


We give an overview of the physical basics, experimental techniques, and selected applications of single molecule fluorescence in biophysical research. The photophysical principles underlying single molecule fluorescence resonance energy transfer (smFRET) experiments are treated. Its application in protein biophysics, in particular protein folding and conformational dynamics associated with enzymatic activity, is outlined with selected model systems.


  • applications;
  • FRET;
  • layouts;
  • photophysical principles;
  • single molecule fluorescence