Single Molecule Biophysics with Fluorescence
Published Online: 15 AUG 2009
Copyright © 2003 by Wiley-VCH Verlag GmbH & Co. KGaA. All rights reserved.
Encyclopedia of Applied Physics
How to Cite
Prummer, M. and Huebner, C. 2009. Single Molecule Biophysics with Fluorescence. Encyclopedia of Applied Physics. .
- Published Online: 15 AUG 2009
We give an overview of the physical basics, experimental techniques, and selected applications of single molecule fluorescence in biophysical research. The photophysical principles underlying single molecule fluorescence resonance energy transfer (smFRET) experiments are treated. Its application in protein biophysics, in particular protein folding and conformational dynamics associated with enzymatic activity, is outlined with selected model systems.
- photophysical principles;
- single molecule fluorescence