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Single-Molecule Fluorescence: Biophysics

  1. Michael Prummer1,
  2. Christian Hübner2

Published Online: 15 DEC 2009

DOI: 10.1002/3527600434.eap687

Encyclopedia of Applied Physics

Encyclopedia of Applied Physics

How to Cite

Prummer, M. and Hübner, C. 2009. Single-Molecule Fluorescence: Biophysics. Encyclopedia of Applied Physics. 627–653.

Author Information

  1. 1

    F. Hoffman-La Roche Ltd., Pharmaceutical Divisions, Discovery Technologies, Basel, Switzerland

  2. 2

    Universitaet zu Lübeck, Institut für Physik, Lübeck, Germany

Publication History

  1. Published Online: 15 DEC 2009

Abstract

The article contains sections titled:

  • 1
    Introduction
  • 2
    Photophysical Basics
    • 2.1
      Energy Levels and Transitions
    • 2.2
      Energy Transfer
    • 2.3
      The Dye Molecule as a Dipole
  • 3
    Techniques
    • 3.1
      Signal-to-Noise Considerations
    • 3.2
      Wide-Field Fluorescence Microscopy Techniques
      • 3.2.1
        Epifluorescence Microscopy
      • 3.2.2
        TIRF Microscopy
    • 3.3
      Confocal Optical Microscopy
  • 4
    Applications
    • 4.1
      Protein Folding
      • 4.1.1
        The Unfolded State
      • 4.1.2
        Single Molecule Folding/Unfolding Transitions
    • 4.2
      Enzymes
      • 4.2.1
        Static and Dynamic Heterogeneity
      • 4.2.2
        Conformational Changes and Catalytic Activity
  • 5
    Conclusions

Keywords:

  • fluorescence light;
  • biophysical technique;
  • single-molecule fluorescence;
  • energy transfer;
  • molecular ruler