Chapter 10. Exploring the Diversity of Heme Enzymes through Directed Evolution

  1. Dr. Susanne Brakmann2,
  2. Prof. Dr. Kai Johnsson3
  1. Patrick C. Cirino,
  2. Prof. Dr. Frances H. Arnold

Published Online: 28 MAR 2003

DOI: 10.1002/3527600647.ch10

Book Title

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Additional Information

How to Cite

Cirino, P. C. and Arnold, F. H. (2003) Exploring the Diversity of Heme Enzymes through Directed Evolution, in Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis (eds S. Brakmann and K. Johnsson), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527600647.ch10

Editor Information

  1. 2

    AG “Angewandte Molekulare Evolution”, Institut für Spezielle Zoologie, Universität Leipzig, Talstraße 33, D-04103 Leipzig, Germany

  2. 3

    Insitute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne, CH-1015 Lausanne, Switzerland

Author Information

  1. Chemical Engineering 210–41, California Institute of Technology, 1201 East California Boulevard, Pasadena, California 91125, USA

Publication History

  1. Published Online: 28 MAR 2003
  2. Published Print: 22 APR 2002

ISBN Information

Print ISBN: 9783527304233

Online ISBN: 9783527600649

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CHAPTER TOOLS

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Keywords:

  • heme proteins;
  • cytochromes P450;
  • peroxidases;
  • HRP;
  • CPO;
  • myoglobin (Mb);
  • Catalase I

Summary

This chapter contains sections titled:

  • Introduction

  • Heme Proteins

  • Cytochromes P450

    • Introduction

    • Mechanism

  • Peroxidases

    • Introduction

    • Mechanism

  • Comparison of P450s and Peroxidases

  • Chloroperoxidase

  • Mutagenesis Studies

    • P450s

    • HRP

    • CPO

    • Myoglobin (Mb)

  • Directed Evolution of Heme Enzymes

    • P450s

    • Peroxidases

    • CPO

    • Catalase I

    • Myoglobin

    • Methods for recombination of P450s

  • Conclusions

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