Chapter 11. Directed Evolution as a Means to Create Enantioselective Enzymes for Use in Organic Chemistry
- Dr. Susanne Brakmann2,
- Prof. Dr. Kai Johnsson3
Published Online: 28 MAR 2003
DOI: 10.1002/3527600647.ch11
Copyright © 2002 Wiley-VCH Verlag GmbH & Co. KGaA
Book Title
Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis
Additional Information
How to Cite
Reetz, M. T. and Jaeger, K.-E. (2003) Directed Evolution as a Means to Create Enantioselective Enzymes for Use in Organic Chemistry, in Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis (eds S. Brakmann and K. Johnsson), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527600647.ch11
Editor Information
- 2
AG “Angewandte Molekulare Evolution”, Institut für Spezielle Zoologie, Universität Leipzig, Talstraße 33, D-04103 Leipzig, Germany
- 3
Insitute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne, CH-1015 Lausanne, Switzerland
Publication History
- Published Online: 28 MAR 2003
- Published Print: 22 APR 2002
ISBN Information
Print ISBN: 9783527304233
Online ISBN: 9783527600649
- Summary
- Chapter
Keywords:
- mutagenesis;
- overexpression;
- enzymes;
- high-throughput screening;
- enantioselectivity;
- lipases;
- transaminase;
- hydantoinase;
- aldolases
Summary
This chapter contains sections titled:
Introduction
Mutagenesis Methods
Overexpression of Genes and Secretion of Enzymes
High-Throughput Screening Systems for Enantioselectivity
Examples of Directed Evolution of Enantioselective Enzymes
Kinetic resolution of a chiral ester catalyzed by mutant lipases
Evolution of a lipase for the stereoselective hydrolysis of a meso-compound
Kinetic resolution of a chiral ester catalyzed by a mutant esterase
Improving the enantioselectivity of a transaminase
Inversion of the enantioselectivity of a hydantoinase
Evolving aldolases which accept both D- and L-glyceraldehydes
Conclusions