Chapter 12. Applied Molecular Evolution of Enzymes Involved in Synthesis and Repair of DNA

  1. Dr. Susanne Brakmann2,
  2. Prof. Dr. Kai Johnsson3
  1. John F. Davidson,
  2. Jon Anderson,
  3. Haiwei Guo,
  4. Daniel Landis,
  5. Prof. Dr. Lawrence A. Loeb

Published Online: 28 MAR 2003

DOI: 10.1002/3527600647.ch12

Book Title

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Additional Information

How to Cite

Davidson, J. F., Anderson, J., Guo, H., Landis, D. and Loeb, L. A. (2003) Applied Molecular Evolution of Enzymes Involved in Synthesis and Repair of DNA, in Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis (eds S. Brakmann and K. Johnsson), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527600647.ch12

Editor Information

  1. 2

    AG “Angewandte Molekulare Evolution”, Institut für Spezielle Zoologie, Universität Leipzig, Talstraße 33, D-04103 Leipzig, Germany

  2. 3

    Insitute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne, CH-1015 Lausanne, Switzerland

Author Information

  1. Department of Pathology, School of Medicine, University of Washington, Box 357705, Seattle, Washington 98195–7705, USA

Publication History

  1. Published Online: 28 MAR 2003
  2. Published Print: 22 APR 2002

ISBN Information

Print ISBN: 9783527304233

Online ISBN: 9783527600649

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Keywords:

  • directed evolution;
  • enzymes;
  • site-directed mutagenesis;
  • PCR mutagenesis;
  • DNA shuffling;
  • random mutagenesis;
  • DNA polymerases;
  • thymidine kinase;
  • thymidylate synthase;
  • DNA alkyltransferase

Summary

This chapter contains sections titled:

  • Introduction

  • Directed Evolution of Enzymes

    • Site-directed mutagenesis

    • Directed evolution

    • Genetic damage

    • PCR mutagenesis

    • DNA shuffling

    • Substitution by oligonucleotides containing random mutations (random mutagenesis)

  • Directed Evolution of DNA polymerases

    • Random mutagenesis of Thermus aquaticus DNA Pol I

    • Random oligonucleotide mutagenesis of Escherichia coli Pol I

  • Directed Evolution of Thymidine Kinase

  • Directed Evolution of Thymidylate Synthase

  • O6-Alkylguanine-DNA Alkyltransferase

  • Discussion

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