Chapter 13. Evolutionary Generation Versus Rational Design of Restriction Endonucleases with Novel Specificity

  1. Dr. Susanne Brakmann2,
  2. Prof. Dr. Kai Johnsson3
  1. Thomas Lanio,
  2. Albert Jeltsch,
  3. Prof. Dr. Alfred Pingoud

Published Online: 28 MAR 2003

DOI: 10.1002/3527600647.ch13

Book Title

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Additional Information

How to Cite

Lanio, T., Jeltsch, A. and Pingoud, A. (2003) Evolutionary Generation Versus Rational Design of Restriction Endonucleases with Novel Specificity, in Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis (eds S. Brakmann and K. Johnsson), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527600647.ch13

Editor Information

  1. 2

    AG “Angewandte Molekulare Evolution”, Institut für Spezielle Zoologie, Universität Leipzig, Talstraße 33, D-04103 Leipzig, Germany

  2. 3

    Insitute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne, CH-1015 Lausanne, Switzerland

Author Information

  1. Institut für Biochemie, Justus-Liebig-Universität, Heinrich-Buff-Ring 58, 35392 Giessen, Germany

Publication History

  1. Published Online: 28 MAR 2003
  2. Published Print: 22 APR 2002

ISBN Information

Print ISBN: 9783527304233

Online ISBN: 9783527600649

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CHAPTER TOOLS

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Keywords:

  • restriction/modification systems;
  • type II restriction endonucleases;
  • rational design;
  • evolutionary design;
  • specificities

Summary

This chapter contains sections titled:

  • Introduction

    • Biology of restriction/modification systems

    • Biochemical properties of type II restriction endonucleases

    • Applications for type II restriction endonucleases

    • Setting the stage for protein engineering of type II restriction endonucleases

  • Design of Restriction Endonucleases with New Specificities

    • Rational design

    • Evolutionary design of extended specificities

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