Chapter 3. Using Evolutionary Strategies to Investigate the Structure and Function of Chorismate Mutases†

  1. Dr. Susanne Brakmann2,
  2. Prof. Dr. Kai Johnsson3
  1. Prof. Dr. Donald Hilvert,
  2. Sean V. Taylor,
  3. Peter Kast

Published Online: 28 MAR 2003

DOI: 10.1002/3527600647.ch3

Book Title

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Additional Information

How to Cite

Hilvert, D., Taylor, S. V. and Kast, P. (2003) Using Evolutionary Strategies to Investigate the Structure and Function of Chorismate Mutases, in Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis (eds S. Brakmann and K. Johnsson), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527600647.ch3

Editor Information

  1. 2

    AG “Angewandte Molekulare Evolution”, Institut für Spezielle Zoologie, Universität Leipzig, Talstraße 33, D-04103 Leipzig, Germany

  2. 3

    Insitute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne, CH-1015 Lausanne, Switzerland

Author Information

  1. ETH Hönggerberg, Laboratorium für Organische Chemie, HCI, F339, CH-8093 Zürich, Schweiz

  1. †

    This article was adapted from a review that appeared in Angew. Chem. 2001, 113, 3408–3436; Angew. Chem Intl. Ed. 2001, 40, 3310–3335

Publication History

  1. Published Online: 28 MAR 2003
  2. Published Print: 22 APR 2002

ISBN Information

Print ISBN: 9783527304233

Online ISBN: 9783527600649

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CHAPTER TOOLS

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Keywords:

  • selection;
  • screening;
  • chorismate mutases;
  • mechanistic studies;
  • structural studies;
  • protein topology;
  • protein design

Summary

This chapter contains sections titled:

  • Introduction

  • Selection versus Screening

    • Classical solutions to the sorting problem

    • Advantages and limitations of selection

  • Genetic Selection of Novel Chorismate Mutases

    • The selection system

    • Mechanistic studies

    • Structural studies

    • Altering protein topology

    • Augmenting weak enzyme activity

    • Protein design

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