Chapter 5. Investigation of Phage Display for the Directed Evolution of Enzymes

  1. Dr. Susanne Brakmann2,
  2. Prof. Dr. Kai Johnsson3
  1. Patrice Soumillion,
  2. Prof. Dr. Jacques Fastrez

Published Online: 28 MAR 2003

DOI: 10.1002/3527600647.ch5

Book Title

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Additional Information

How to Cite

Soumillion, P. and Fastrez, J. (2003) Investigation of Phage Display for the Directed Evolution of Enzymes, in Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis (eds S. Brakmann and K. Johnsson), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527600647.ch5

Editor Information

  1. 2

    AG “Angewandte Molekulare Evolution”, Institut für Spezielle Zoologie, Universität Leipzig, Talstraße 33, D-04103 Leipzig, Germany

  2. 3

    Insitute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne, CH-1015 Lausanne, Switzerland

Author Information

  1. Laboratoire de Biochimie Physique et de Biopolymères, Université Catholique de Louvain Place L. Pasteur, 1.Bte 1B, B-1348 Louvain-la-Neuve, Belgium

Publication History

  1. Published Online: 28 MAR 2003
  2. Published Print: 22 APR 2002

ISBN Information

Print ISBN: 9783527304233

Online ISBN: 9783527600649

SEARCH

CHAPTER TOOLS

Get PDF (345K)

Keywords:

  • phage display;
  • enzymes;
  • expression vectors;
  • libaries;
  • mutants;
  • phage-enzymes

Summary

This chapter contains sections titled:

  • Introduction

  • The Phage Display

  • Phage Display of Enzymes

    • The expression vectors

    • Phage-enzymes

  • Creating Libraries of Mutants

  • Selection of Phage-enzymes

    • Selection for binding

    • Selection for catalytic activity

  • Conclusions

Get PDF (345K)