Chapter 6. Directed Evolution of Binding Proteins by Cell Surface Display: Analysis of the Screening Process

  1. Dr. Susanne Brakmann2,
  2. Prof. Dr. Kai Johnsson3
  1. Prof. Dr. K. Dane Wittrup

Published Online: 28 MAR 2003

DOI: 10.1002/3527600647.ch6

Book Title

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Additional Information

How to Cite

Wittrup, K. D. (2003) Directed Evolution of Binding Proteins by Cell Surface Display: Analysis of the Screening Process, in Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis (eds S. Brakmann and K. Johnsson), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527600647.ch6

Editor Information

  1. 2

    AG “Angewandte Molekulare Evolution”, Institut für Spezielle Zoologie, Universität Leipzig, Talstraße 33, D-04103 Leipzig, Germany

  2. 3

    Insitute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne, CH-1015 Lausanne, Switzerland

Author Information

  1. Dept. Chemical Engineering & Div. Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

Publication History

  1. Published Online: 28 MAR 2003
  2. Published Print: 22 APR 2002

ISBN Information

Print ISBN: 9783527304233

Online ISBN: 9783527600649

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CHAPTER TOOLS

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Keywords:

  • library construction;
  • mutagenesis;
  • expression;
  • mutant isolation;
  • labeling;
  • screening

Summary

This chapter contains sections titled:

  • Introduction

  • Library Construction

    • Mutagenesis

    • Expression

  • Mutant Isolation

    • Differential labeling

    • Screening

Get PDF (200K)