Chapter 7. Yeast n-Hybrid Systems for Molecular Evolution

  1. Dr. Susanne Brakmann2,
  2. Prof. Dr. Kai Johnsson3
  1. Brian T. Carter,
  2. Hening Lin,
  3. Prof. Dr. Virginia W. Cornish

Published Online: 28 MAR 2003

DOI: 10.1002/3527600647.ch7

Book Title

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Additional Information

How to Cite

Carter, B. T., Lin, H. and Cornish, V. W. (2003) Yeast n-Hybrid Systems for Molecular Evolution, in Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis (eds S. Brakmann and K. Johnsson), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527600647.ch7

Editor Information

  1. 2

    AG “Angewandte Molekulare Evolution”, Institut für Spezielle Zoologie, Universität Leipzig, Talstraße 33, D-04103 Leipzig, Germany

  2. 3

    Insitute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne, CH-1015 Lausanne, Switzerland

Author Information

  1. Columbia University, Department of Chemistry, 3000 Broadway, MC 3167, New York, NY 10027-6948, USA

Publication History

  1. Published Online: 28 MAR 2003
  2. Published Print: 22 APR 2002

ISBN Information

Print ISBN: 9783527304233

Online ISBN: 9783527600649

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CHAPTER TOOLS

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Keywords:

  • yeast two-hybrid assay;
  • protein-protein interactions;
  • protein-DNA interactions;
  • protein-RNA interactions;
  • small molecule

Summary

This chapter contains sections titled:

  • Introduction

  • Technical Considerations

    • Yeast two-hybrid assay

    • Alternative assays

  • Applications

    • Protein-protein interactions

    • Protein-DNA interactions

    • Protein-RNA interactions

    • Protein-small molecule interactions

  • Conclusion

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