Chapter 9. Engineering Protein Evolution
- Dr. Susanne Brakmann2,
- Prof. Dr. Kai Johnsson3
Published Online: 28 MAR 2003
DOI: 10.1002/3527600647.ch9
Copyright © 2002 Wiley-VCH Verlag GmbH & Co. KGaA
Book Title
Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis
Additional Information
How to Cite
Lutz, S. and Benkovic, S. J. (2003) Engineering Protein Evolution, in Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis (eds S. Brakmann and K. Johnsson), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527600647.ch9
Editor Information
- 2
AG “Angewandte Molekulare Evolution”, Institut für Spezielle Zoologie, Universität Leipzig, Talstraße 33, D-04103 Leipzig, Germany
- 3
Insitute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne, CH-1015 Lausanne, Switzerland
Publication History
- Published Online: 28 MAR 2003
- Published Print: 22 APR 2002
ISBN Information
Print ISBN: 9783527304233
Online ISBN: 9783527600649
- Summary
- Chapter
Keywords:
- protein evolution;
- gene duplication;
- circular permutation;
- oligomerization;
- gene fusion;
- exon shuffling;
- protein folding;
- modular
Summary
This chapter contains sections titled:
Introduction
Mechanisms of Protein Evolution in Nature
Gene duplication
Tandem duplication
Circular permutation
Oligomerization
Gene fusion
Domain recruitment
Exon shuffling
Engineering Genes and Gene Fragments
Protein fragmentation
Rational swapping of secondary structure elements and domains
Combinatorial gene fragment shuffling
Modular recombination and protein folding
Rational domain assembly engineering zinc fingers
Combinatorial domain recombination exon shuffling
Gene Fusion From Bi- to Multifunctional Enzymes
End-to-end gene fusions
Gene insertions
Modular design in multifunctional enzymes
Perspectives