Standard Article

Denaturation of DNA

Nucleic Acids

  1. R. D. Blake

Published Online: 15 SEP 2006

DOI: 10.1002/3527600906.mcb.200200014

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Blake, R. D. 2006. Denaturation of DNA . Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. University of Maine, Orono, USA

Publication History

  1. Published Online: 15 SEP 2006

Abstract

Denatured DNA and RNA are disordered nonnative states brought about by any of the many means of disturbing noncovalent bonds supporting their native conformations: heat, nonaqueous denaturing solvents, low or high pHs, low ionic strengths. The denaturation process is generally reversible.

Keywords:

  • Degradation;
  • Stacking Forces;
  • Melting Curve;
  • TM ;
  • Helix–Coil Transition