Standard Article

Expression Systems for DNA Processes

Biotechnology

  1. Ka-Yiu San,
  2. George N. Bennett

Published Online: 15 SEP 2006

DOI: 10.1002/3527600906.mcb.200300074

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

San, K.-Y. and Bennett, G. N. 2006. Expression Systems for DNA Processes. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. Rice University, Houston, Texas, USA

Publication History

  1. Published Online: 15 SEP 2006

Abstract

The production of a specific protein by recombinant DNA technology entails cloning the gene that encodes the protein from the desired organism, forming a suitable genetic construct such that the gene can be expressed in a host organism, and introduction and maintenance of the construct in the host to allow the production of an adequate yield of functional protein. The ability to take a gene from any organism and express it in adequate quantity in a system permitting the analysis of the structure and function of the protein encoded by the gene has great importance in the research laboratory. Physical and enzymatic features of the protein encoded by a newly discovered gene can be studied, and its functional location in cells or tissues can be determined, in an effort to better define its biological function. Industrial applications include the production of therapeutic and diagnostic proteins having great impact in the field of medicine, and the production of stable bulk enzymes for the food and specialty chemical industries.

Keywords:

  • Antibiotic Resistance;
  • Bacteriophage;
  • Coding Region;
  • Codon;
  • Fusion Protein;
  • Inducible Promoter;
  • Partition;
  • Posttranslational Processing;
  • Promoter;
  • Protease;
  • Ribosome Binding Site;
  • RNA Secondary Structure;
  • Secretion;
  • Virus