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Flow Cytometry

Cell Biology

  1. Michael G. Ormerod

Published Online: 15 SEP 2006

DOI: 10.1002/3527600906.mcb.200300144

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Ormerod, M. G. 2006. Flow Cytometry. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. Reigate, UK

Publication History

  1. Published Online: 15 SEP 2006

Abstract

A flow cytometer measures between one and thirteen or more parameters on single cells. The flow system is used to deliver cells to the point of measurement. In most instruments, the cells are interrogated by laser light. Measurements are made of scattered light and of fluorescence, either from the cells or, more commonly, from labelled probes. Sub-populations of cells may also be physically sorted for further study. The article briefly describes the main attributes of the technology and its applications in clinical areas and in mammalian biology.

Keywords:

  • Flow Cytometry;
  • Cell Sorting