Flow Cytometry

A flow cytometer measures between one and thirteen or more parameters on single cells. The flow system is used to deliver cells to the point of measurement. In most instruments, the cells are interrogated by laser light. Measurements are made of scattered light and of fluorescence, either from the cells or, more commonly, from labelled probes. Sub-populations of cells may also be physically sorted for further study. The article briefly describes the main attributes of the technology and its applications in clinical areas and in mammalian biology.

Keywords: Flow Cytometry; Cell Sorting