Fluorescence Correlation Spectroscopy (FCS)
Structural Determination Techniques (DNA, RNA and Protein)
Published Online: 15 SEP 2006
Copyright © 2006 Wiley-VCH Verlag GmbH & Co. KGaA. All rights reserved.
Reviews in Cell Biology and Molecular Medicine
How to Cite
Pramanik, A. and Widengren, J. 2006. Fluorescence Correlation Spectroscopy (FCS). Reviews in Cell Biology and Molecular Medicine. .
- Published Online: 15 SEP 2006
In Fluorescence Correlation Spectroscopy (FCS), fluctuations of the fluorescence intensity of fluorophore-labeled molecules, excited by a sharply focused laser beam, are observed. Owing to high sensitivity, detection at the level of single molecules is possible. Thermodynamic fluctuations can thus be revealed, which would be difficult to detect at a macroscopic level. The technique can, in principle, offer information about any molecular dynamic process in the nanosecond time range and longer, manifesting itself as a change in fluorescence intensity without the need for any perturbation or synchronization of the system studied. The specific features of FCS make it a versatile tool for biomolecular studies, including determination of translational and rotational diffusion, concentration and density of molecules, chemical kinetics, and binding reactions. In this review, an overview of the theory and the typical experimental setup for FCS measurements will be given, along with some examples of how FCS can be used for molecular dynamic studies.
- Fluorescence Correlation Spectroscopy (FCS);
- Brownian Motion;
- Single Molecule Detection;
- Molecular Interactions;
- Drug Screening