Mass Spectrometry, High Speed DNA Fragment Sizing
Structural Determination Techniques (DNA, RNA and Protein)
Published Online: 15 SEP 2006
Copyright © 2006 Wiley-VCH Verlag GmbH & Co. KGaA. All rights reserved.
Reviews in Cell Biology and Molecular Medicine
How to Cite
Chen, C.-H. 2006. Mass Spectrometry, High Speed DNA Fragment Sizing. Reviews in Cell Biology and Molecular Medicine. .
- Published Online: 15 SEP 2006
Recently, high-speed DNA fragment sizing has been in critical need due to its important application on genomic function, DNA sequencing, disease diagnosis, DNA typing for forensic use, and microbial analysis for bioagent identification. Mass spectrometry has played a key role in rapid DNA sizing. This article discusses the fundamental principles of mass spectrometry for DNA analysis and the advantages and disadvantages of mass spectrometry compared to other approaches. It also gives a brief historical review of the development of mass spectrometry for DNA analysis. Applications in DNA sequencing, genetic disease diagnosis, and DNA finger printing are also presented.
- Matrix-assisted Laser Desorption and Ionization (MALDI);
- Time-of-Flight Mass Spectrometer (TOF);
- Gel Electrophoresis;
- DNA Hybridization;
- DNA Sequencing