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Mass Spectrometry, High Speed DNA Fragment Sizing

Structural Determination Techniques (DNA, RNA and Protein)

  1. Chung-Hsuan Chen

Published Online: 15 SEP 2006

DOI: 10.1002/3527600906.mcb.200400008

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Chen, C.-H. 2006. Mass Spectrometry, High Speed DNA Fragment Sizing. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

Publication History

  1. Published Online: 15 SEP 2006

Abstract

Recently, high-speed DNA fragment sizing has been in critical need due to its important application on genomic function, DNA sequencing, disease diagnosis, DNA typing for forensic use, and microbial analysis for bioagent identification. Mass spectrometry has played a key role in rapid DNA sizing. This article discusses the fundamental principles of mass spectrometry for DNA analysis and the advantages and disadvantages of mass spectrometry compared to other approaches. It also gives a brief historical review of the development of mass spectrometry for DNA analysis. Applications in DNA sequencing, genetic disease diagnosis, and DNA finger printing are also presented.

Keywords:

  • Matrix-assisted Laser Desorption and Ionization (MALDI);
  • Time-of-Flight Mass Spectrometer (TOF);
  • Gel Electrophoresis;
  • DNA Hybridization;
  • DNA Sequencing