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Reporter Gene System: Green Fluorescent Protein

Proteins, Peptides and Amino Acids

  1. Boon Chuan Low,
  2. Zhiyuan Gong

Published Online: 15 SEP 2006

DOI: 10.1002/3527600906.mcb.200400013

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Chuan Low, B. and Gong, Z. 2006. Reporter Gene System: Green Fluorescent Protein. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. National University of Singapore, Singapore

Publication History

  1. Published Online: 15 SEP 2006

Abstract

Green fluorescent protein (GFP) possesses unique biochemical and biophysical properties that make it a popular and powerful tool for both basic and applied research. Originally isolated from jellyfish, its gene can be introduced to various host species and results in a stable protein that can form matured chromophore without the need of other cofactors and enzymes. Various wildtype and mutant forms of GFP have been identified through protein engineering or the search for similar proteins in other species such as reef corals and their relatives. With unique properties, they offer excellent markers for monitoring various biological processes in a single cell or whole organism. These include viewing of the internal structure and protein movement as well as studies of protein–protein interaction. As a transgene, it is also very useful for monitoring developmental process and as a promising source of genetically modified ornamentals. Coupled with advanced bioimaging methods, GFP and its variants are expected to continue to provide new insights into many biological processes.

Keywords:

  • Cell Signaling;
  • Chromophore;
  • FACS;
  • Fluorescence;
  • FRET;
  • Transgenic Organisms