Mass Spectrometry-based Methods of Proteome Analysis
Proteins, Peptides and Amino Acids
Published Online: 15 SEP 2006
Copyright © 2006 Wiley-VCH Verlag GmbH & Co. KGaA. All rights reserved.
Reviews in Cell Biology and Molecular Medicine
How to Cite
Zybailov, B. L. and Washburn, M. P. 2006. Mass Spectrometry-based Methods of Proteome Analysis. Reviews in Cell Biology and Molecular Medicine. .
- Published Online: 15 SEP 2006
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Proteomics aims to identify, characterize, and map gene functions at the protein level for whole cells or organisms. A typical experimental scheme for a large-scale proteomics inquiry involves fractionation of a complex protein mixture by electrophoretic or chromatographic means followed by subsequent identification of the components in the individual fractions by mass spectrometry. Owing to continuous and rapid improvement in instrument sensitivity, throughput capacity, software versatility, and techniques of statistical validation of the data, mass spectrometry–based approaches are becoming mainstream methods in a proteome analysis.
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