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Mass Spectrometry-based Methods of Proteome Analysis

Proteins, Peptides and Amino Acids

  1. Boris L. Zybailov,
  2. Michael P. Washburn

Published Online: 15 SEP 2006

DOI: 10.1002/3527600906.mcb.200400028

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Zybailov, B. L. and Washburn, M. P. 2006. Mass Spectrometry-based Methods of Proteome Analysis. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. Stowers Institute for Medical Research, Kansas City, MO, USA

Publication History

  1. Published Online: 15 SEP 2006

This is not the most recent version of the article. View current version (27 JUL 2015)

Abstract

Proteomics aims to identify, characterize, and map gene functions at the protein level for whole cells or organisms. A typical experimental scheme for a large-scale proteomics inquiry involves fractionation of a complex protein mixture by electrophoretic or chromatographic means followed by subsequent identification of the components in the individual fractions by mass spectrometry. Owing to continuous and rapid improvement in instrument sensitivity, throughput capacity, software versatility, and techniques of statistical validation of the data, mass spectrometry–based approaches are becoming mainstream methods in a proteome analysis.

Keywords:

  • 2D Page;
  • MS/MS;
  • MudPIT;
  • Quantitative Proteomics