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Mammalian Cell Culture Methods

Cell Biology

  1. Dieter F. Hülser

Published Online: 15 SEP 2006

DOI: 10.1002/3527600906.mcb.200400044

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Hülser, D. F. 2006. Mammalian Cell Culture Methods. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. University of Stuttgart, Stuttgart, Germany

Publication History

  1. Published Online: 15 SEP 2006


Mammalian cell cultures originate from tissue explants or cell suspensions as primary cell cultures that can be subcultured with a limited life span. By transformation, these cells might lose some of their original properties and establish permanent growth. Many of these continuous cell lines are aneuploid and genetically unstable, nevertheless, our knowledge of molecular, physiological, biochemical, and biophysical properties of cells is notably based on investigations with such cell lines. Since the synthesis of various bioproducts such as vaccines, monoclonal antibodies, enzymes, and hormones is accomplished with cell cultures, many efforts were made to develop and improve cell culture technology. Besides this impetus, cancer research also stimulated the progress of cell culture methods as can be seen with the development of three-dimensionally growing cultures. These cultures provided a better understanding of tumor invasion and revealed the importance of the extracellular matrix for physiological regulations of cell–cell interactions that cannot be observed with monolayer or suspension cultures. This knowledge helped to improve the cultivation of cells that are used in clinical treatments as is the case for wound healing with implantation of epidermis or for defect organs such as liver, which can be supported with bioartificial organs during a temporary extracorporal bypass.


  • Cell Cloning;
  • Cell Cycle;
  • Cell Line;
  • Cell Strain;
  • Continuous Cell Line;
  • Primary Culture;
  • Serum;
  • Suspension Culture;
  • Tissue Culture