Published Online: 15 SEP 2006
Copyright © 2006 Wiley-VCH Verlag GmbH & Co. KGaA
Encyclopedia of Molecular Cell Biology and Molecular Medicine
How to Cite
Sano, T. and Cantor, C. R. 2006. Immuno-PCR. Encyclopedia of Molecular Cell Biology and Molecular Medicine.
- Published Online: 15 SEP 2006
Immuno-PCR is an antigen detection system, in which the polymerase chain reaction (PCR) is used to amplify a segment of marker DNA that has been attached specifically to antigenantibody complexes. Because of the enormous amplification capability and specificity of PCR, immuno-PCR allows considerable enhancement in detection sensitivity of a specific antigen over conventional antigen detection systems, such as enzyme-linked immunosorbent assays and radioimmunoassays. A variety of superior characteristics and the versatility of immuno-PCR, including high but controllable sensitivity, simplicity, and easy contamination control, offer great promise for its application in various aspects of biological and medical sciences, as well as clinical diagnostics and forensic medicine.
Keywords: Avidin; Biotin; Biotinylation; Protein A; Streptavidin