Liposome Gene Transfection

Abstract

Varied results have been obtained using cationic liposomes for in vivo delivery. Furthermore, optimization of cationic liposomal complexes for in vivo applications is complex involving many diverse components. These components include nucleic acid purification, plasmid design, formulation of the delivery vehicle, administration route and schedule, dosing, detection of gene expression, and others. This chapter will also focus on optimization of the delivery vehicle formulation. These formulation issues include morphology of the complexes, lipids used, flexibility versus rigidity, colloidal suspension, overall charge, serum stability, half-life in circulation, biodistribution, delivery to and dissemination throughout target tissues. Broad assumptions have been frequently made on the basis of the data obtained from focused studies using cationic liposomes. However, these assumptions do not necessarily apply to all delivery vehicles and, most likely, do not apply to many liposomal systems when considering these other key components that influence the results obtained in vivo. Optimizing all components of the delivery system is pivotal and will allow broad use of liposomal complexes to treat or cure human diseases or disorders. This chapter will highlight the features of liposomes that contribute to successful delivery, gene expression, and efficacy.