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Principles and Applications of Embryogenomics

Systems Biology

  1. Vincent Van Buren

Published Online: 15 MAY 2012

DOI: 10.1002/3527600906.mcb.200400082.pub2

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Van Buren, V. 2012. Principles and Applications of Embryogenomics. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. Texas A & M Health Science Center College of Medicine, Systems Biology and Translational Medicine, Temple, TX, USA

Publication History

  1. Published Online: 15 MAY 2012

Abstract

Embryogenomics, or developmental genomics, is defined as a systematic analysis of embryo development with large-scale approaches. This systematic analysis permits consideration of the system as a whole by keeping whole-genome sequences and a complete gene catalog in perspective. By merging genomics and developmental biology, embryogenomics contributes to both disciplines: large-scale methodologies and genome-wide concepts are contributed to developmental biology, and the materials and knowledge of genes specifically expressed in embryos are contributed to genomics. In this chapter the history and recent strides in this effort are reviewed, and discussions included of the genome projects, complementary DNA (cDNA) library construction, and large-scale experiments and analysis for model organisms that have the greatest impact on the field of embryology. Emphasis will be placed on progress in mouse embryogenomics, as this provides the basis of discussion for what is arguably the most accessible model organism with a developmental plan that has high similarity to that of humans. Finally, the status of current technologies as they relate to embryogenomics, the obstacles to overcome, and the future aims for this new field are discussed. Large-scale approaches to developmental biology require some special considerations in this discussion, as compared to approaches using samples from adult organisms. This is due to the relatively small size of tissue samples and the temporal-spatial migration and proliferation of cells in embryonic tissues of interest.

Keywords:

  • BAC;
  • cDNA;
  • DIGE (or 2-D DIGE);
  • DNA Microarray;
  • Embryo;
  • Genome;
  • Genomics;
  • Hybridization;
  • ISH;
  • Next-generation sequencing;
  • PCR;
  • Q-PCR;
  • SAGE;
  • Transcriptome