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Vector System: Plasmid DNA

Biotechnology

  1. Rajkumar Banerjee1,
  2. Leaf Huang2

Published Online: 15 SEP 2006

DOI: 10.1002/3527600906.mcb.200500030

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Banerjee, R. and Huang, L. 2006. Vector System: Plasmid DNA. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. 1

    Indian Institute of Chemical Technology, Hyderabad, India

  2. 2

    University of Pittsburgh, Pittsburgh, PA 15213

Publication History

  1. Published Online: 15 SEP 2006

Abstract

In gene therapy, nonviral gene delivery has earned a special position on its own right. This delivery system enjoys several favorable properties, such as, the delivery efficiency is not limited by the size of the genetic cargo, and it is less immunogenic and less toxic than their viral counterpart. One of the methods of delivery is direct injection of naked DNA to the organs of interest. Plasmid DNAs (pDNA), which carry recombinant genes of interest, are used for introducing genes to cells and organs. Various physical methods, for example, gene gun and electroporation, increase the efficiency of the naked DNA incorporation. Lipid-based vectors have the advantage of protecting the DNA against degradation by endogenous DNase in vivo, and can be targeted to a specific cellular site in some special cases. Varieties of lipid-based systems have been designed, mostly containing cationic lipids of different structures. Second-generation vectors containing polymers are more stable and more targetable than the first generation vectors. Although significant progress has been made, nonviral vectors are still limited in their efficiency and by some cytotoxicity inherited in the bacterial pDNA, which hosts the gene of interest. Understanding mechanisms and means to overcome the cellular barriers for the DNA delivery will undoubtedly promote further development of pDNA mediated gene delivery.

Keywords:

  • Gene Therapy;
  • Lipoplex;
  • Lipopolyplex;
  • Nonviral Gene Delivery;
  • Polyplex