Regulome

Abstract

The regulome is defined as the complete set of regulons, where a regulon is the complete set of genes positively and negatively regulated by a specific regulatory protein. We propose the study of the regulome in mouse by analysis of 2500 known or predicted transcription factors, by generating one clone mouse with each TAP-tagged TF, followed by the study of the DNA binding sites of the TF by chromatin immunoprecipitation (ChIP) and the protein partners by proteomics, in several cellular types in vitro, and in different tissues and developmental stages in vivo. The integration of those data should provide the complete plan of mammalian transcriptional regulation.