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Total Analysis Systems, Micro

Biotechnology

  1. Shigeori Takenaka

Published Online: 15 SEP 2006

DOI: 10.1002/3527600906.mcb.200500046

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Takenaka, S. 2006. Total Analysis Systems, Micro. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. Kyushu Institute of Technology, Kitakyushu-shi, Japan

Publication History

  1. Published Online: 15 SEP 2006

Abstract

The investigations concerned with downsizing and integration of chemical experiments and processes on a chip attract great attention. These researches have not only brought about integration or miniaturization of whole chemical processes to the scale of a semiconductor integrated circuit but also provided several advantages such as the reduction of chemicals, waste, reaction space, and reaction and measurement time. These devices generally exploit micro size fluidic lines on the flat surface made of glass or plastic. The device thus obtained is called a microfluidic device and the system is called μTAS (Micro Total Analysis Systems) or Lab-on-a-Chip. Such devices have been evolving in the biotechnology area, especially as a gel electrophoretic DNA separation module for DNA sequencing. On the other hand, the research fields connected with the integration of many different DNA sequences on a small space also have been actively explored to provide a tool for high-throughput analysis of events associated with genes. This system is called a DNA chip and helps to collect genetic information closely associated with many genetic diseases. A DNA chip is expected to provide a future gene diagnosis method, since this research combined with the microfluidic system has been studied to develop a rapid pretreatment method of the sample. An electrochemical detecting method is more suitable for such a miniature system than the conventional fluorescent one currently in use.

Keywords:

  • Capillary Gel Electrophoresis;
  • DNA Probe;
  • Hybridization;
  • Polymerase Chain Reaction (PCR);
  • Single Nucleotide Polymorphism (SNP)